微重力环境下体外构建组织工程化椎间盘  

Construction of tissue engineered intervertebral disc under microgravity culture

作　　者：刘洋[1] 朱书涛[1] 李国军[1] 王晓[1] 张永乐[1] 

机构地区：[1]河南大学淮河医院骨科,开封475000

出　　处：《中国矫形外科杂志》2013年第3期291-295,共5页Orthopedic Journal of China

基　　金：河南省科技厅重点攻关项目(编号:112102310286)

摘　　要：[目的]利用微重力培养环境,构建高质量组织工程化椎间盘。[方法]选用成年新西兰大白兔椎间盘细胞作为种子细胞,复合聚乳酸-羟基乙酸(PLGA)支架,分别在微重力培养环境和普通培养板环境下培养,利用倒置显微镜、MTT比色法、扫描电镜、组织学观察椎间盘细胞、支架和复合结构质量。[结果]体外单层培养的椎间盘细胞呈多角形符合传代要求,微重力环境比普通培养环境能更好地促进椎间盘细胞的增殖,同时椎间盘细胞在支架内分布更加均匀。[结论]微重力培养环境更适于椎间盘细胞在三维培养结构中均匀增殖,有利于构建高质量组织工程化椎间盘用于深入研究。[ Objective] To construct high quality tissue engineered intervertebral disc under microgravity culture. [ Methods ] Nucleus pulposus cells were isolated from intervertebral disc of rabbit, and the cells were seeded onto the polylactic -glycolic acid scaffold, then the cell - scaffold composites were cultured in both microgravity and ordinary environment for 4 weeks. The cell - scaffold composites were observed in vitro by phase - contrast microscopy, MTI＇ assay, histology and electron microscopy scanning. [ Results ] The rabbit nucleus pulposus cells displayed a polygonal shape in primary monolayer culture. The cells that adhered to the scaffold increased significantly and distributed more uniformly under microgravity compared with in ordinary environment. [ Conclusion] The microgravity environment will be helpful for the culture of tissue engineered intervertebral disc.

关 键 词：微重力 细胞培养 椎间盘 组织工程 髓核细胞 

分 类 号：R318.01[医药卫生—生物医学工程]