激流灌注式生物反应器培养猪繁殖与呼吸综合征活疫苗(R98株)工艺的研究  

Trial-manufacture of porcine reproductive and respiratory syndrome live vaccine with suspension and perfusion bioreactor

作　　者：郑朝朝[1] 邱贞娜 李建丽[1] 郁宏伟[1] 梁武[1] 杨保收[1] 

机构地区：[1]瑞普(保定)生物药业有限公司,河北保定071000

出　　处：《中国预防兽医学报》2013年第2期107-109,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：河北省农业科技成果转化资金项目(12820420D)

摘　　要：为建立猪繁殖与呼吸综合征(PRRS)疫苗的大规模生产工艺,本研究应用工作体积为100 L的激流灌注式生物反应器培养Marc-145细胞,接种PRRS病毒R98株(PRRSV-R98),进行病毒培养,结果显示,培养6 d细胞数量可增殖5倍～7倍,单次病毒收获量相当于3 000个～5 000个10 L转瓶,而且培养的PRRSV-R98各项指标均符合规程要求。本研究在国内首次建立了应用100 L激流灌注式生物反应器制备PRRS活疫苗的生产工艺,为规模化培养工艺的推广与使用提供了技术参数。In the present study, the 100L volume of suspension and perfusion bioreactor was applied to prepare Marc-145 cells and the live vaccine was produced with porcine reproductive and respiratory syndrome virus （PRRSV） R98 strain. The results showed that number of cells increased by 5 to 7 folds in 6 days and the yield from single virus harvest was equivalent to 3,000 to 5,000 l0 L flasks, and the PRRSV R98 strain satisfies all the biological qualifications and requirements. Our study is the first report on applying 100 L volume of torrent in suspension and perfusion bioreactor in the production of PRRSV and it provided the basis for large scale cell culture in vaccine production.

关 键 词：生物反应器 规模化培养 猪繁殖与呼吸综合征活疫苗 

分 类 号：S852.65[农业科学—基础兽医学]