I型鸭肝炎病毒在鸭胚成纤维细胞系中的增殖特性研究  

Proliferation characteristics of duck hepatitis virus type 1 in duck embryo fibroblast cells

作　　者：付玉志[1,2] 张洪辉 李传峰[1] 陈宗艳[1] 王超[1] 陈铁桥[2] 刘光清[1] 

机构地区：[1]中国农业科学院上海兽医研究所,上海200241 [2]湖南农业大学动物医学院,湖南长沙410128 [3]濉溪县动物检疫站,安徽淮北235103

出　　处：《中国预防兽医学报》2013年第2期110-113,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(30870114;31101848);公益性农业科研专项(201003012);国家863计划(2011AA10A200)

摘　　要：为研究I型鸭肝炎病毒(DHV-1)在鸭胚成纤维细胞系(DEF-h)中的增殖规律,本研究将DHV-1野毒株(ZJ-08株)接种DEF-h细胞,连续传代3次后,观察病毒对细胞的致病变效应(CPE),并对病毒核酸及其蛋白的表达和病毒增殖特性进行了鉴定。研究结果显示,DHV ZJ-08株能够在DEF-h中有效增殖,并产生典型的CPE,病毒蛋白在细胞中也获得了良好表达,并与DHV-1特异性抗体发生反应。一步生长曲线显示ZJ-08株病毒感染DEF-h 12 h开始增殖,36 h～60 h进入快速增殖期,72 h达到峰值,TCID50为10-4.3/mL。本实验为进一步的DHV-1的致病机理和细胞灭活疫苗的研制奠定了基础。To explore the proliferation characteristics of duck hepatitis virus type 1 （DHV-1） in duck embryo fibroblast cell line （DEF-h）, DEF-h cells were inoculated with DHV-1 ZJ-08 strain. After passaged three times, CPE could be observed clearly at 36 hpi. The results of RT-PCR, IFA and western blot showed that virual proteins of DHV-1 were expressed well in DEF-h cells, and could reacted to the specific antibody against DHV-I. One step growth curve revealed that DHV-1 began to proliferate at 12 hpi, and reached a rapid proliferation stage at 36 hpi. The virus titre of DHV-1 reached peak at 72 hpi. This study provide some valuable information for further exploring the pathogenesis mechanism of DHV-1 and the vaccine production.

关 键 词：Ⅰ型鸭肝炎病毒 成纤维细胞 一步生长曲线 

分 类 号：S852.65[农业科学—基础兽医学]