西南地区猪肠外大肠杆菌毒力基因检测及耐药性研究  

Virulence gene detection and antibiotic sensitivity of swine extraintestinal Eschericha coli from southwest China

作　　者：曾博[1] 王红宁[1] 邹立扣[1] 徐昌文[1] 张安云[1] 杨鑫[1] 周英顺[1] 黄康[1] 

机构地区：[1]f四川大学生命科学学院生物资源与生态环境教育部重点实验室/动物疫病防控与食品安全四川省重点实验室,四川成都610064

出　　处：《中国预防兽医学报》2013年第2期126-129,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技支撑计划(2007BAD51B05、2006BAD14B05-05)

摘　　要：为了解规模化猪场肠外致病性大肠杆菌(ExPEC)的毒力基因与耐药性的流行情况,本实验室于2008年～2011年间对中国西南地区61个不同规模化猪场的427份组织病料进行细菌检验。采用菌落形态、显微观察和16SrDNA PCR测序鉴定分离菌,共检出79株(18.5%)ExPEC。对小鼠致病性试验结果显示其中50个分离株具有致病性,致病菌为66.3%。此外,对其8种毒力基因PCR检测结果表明astA、escV、invE、Stx2e 4种基因检出率分别为48%、44%、10%、4%,其他4种毒力基因未检出。18种药物的药敏试验结果显示头孢噻呋(7.6%)、氨苄西林/舒巴坦(11.4%)、阿莫西林/棒酸(16.4%)相对敏感,其他药耐药率均高于24%,多重耐药严重。该研究结果对近年来猪源大肠杆菌病的防控和临床用药提供了实验依据。To investigate the virulence genes and antibiotic susceptibility of extraintestinal pathogenic Eschericfiia coli （ExPEC） in Southwest China, a total of 427 tissue samples were collected from dead or dying pigs in 61 pig farms during 2008 to 2011 and 79 isolates of ExPEC were isolated which were identified by colony morphology, microscopic observation and 16S rDNA sequencing, of which 50 isolates were proved to be pathogenic to mice. In addition, the virulence genes were detected by PCR and the results showed that astA, escV, invE, Stx2e were positive in the 50 isolates by 48%, 44%, 10% and 4%, respectively. Furthermore, The isolates were tested on 18 antibiotics and the results demonstrated that Ceftiofur （7.6%）, Ampicillin/sulbactam （11.4%）, Amoxicillin/clavulanic acid （16.4%） were more sensitive than other 15 antibiotics （〉24%）, and most of the ExPEC were multi-drug resistance. This study was important to swine colibacillosis prevention, and provide reference for clinical treatment.

关 键 词：肠外致病性大肠杆菌 猪 毒力基因 药敏实验 

分 类 号：S852.61[农业科学—基础兽医学]