补肾中药合剂促进成骨细胞功能的机制研究  

The mechanism of kidney-reinforcing chinese herbal mixture in the promoting osteoblast function in vitro

作　　者：郭吕华[1] 杨岚[1] 杜发亮[1] 黄江勇[1] 方颖[1] 刘文静[1] 施斌[2] 

机构地区：[1]广州医学院口腔医院修复、种植科,510140 [2]武汉大学口腔医院种植科

出　　处：《实用口腔医学杂志》2013年第1期56-60,共5页Journal of Practical Stomatology

基　　金：广东省中医药局立项资助课题(编号:20111264);广州市荔湾区科技局立项资助课(编号:20111213053)

摘　　要：目的:探讨补肾中药合剂对体外培养的人成骨细胞(hFOB 1.19)功能影响的分子机制。方法:分别对低剂量、高剂量补肾中药血清和5%空白对照组血清培养的成骨细胞进行碱性磷酸酶(ALP)测试,并通过酶联免疫吸附实验分别检测其成骨细胞骨钙素(OC)的分泌量。采用蛋白质印迹法分析补肾中药合剂含药血清对骨保护素(OPG)、骨桥蛋白(OPN)和核因子κB受体活化因子配体(RANKL)蛋白表达水平的影响。结果:补肾中药合剂含药血清能提高hFOB 1.19细胞中的ALP活性;作用96 h后,能促进hFOB 1.19细胞分泌OC,均具有剂量依赖关系;在蛋白表达水平上,补肾中药合剂含药血清能明显上调人成骨细胞的OPG、OPN,并抑制其RANKL的表达。结论:补肾中药合剂含药血清可增张成骨细胞的成骨功能,其机制可能与影响OPG/RANK/RANKL骨代谢信号调节系统有关。Objective： To study the effects of the medicated serum of a kidney-reinforcing chinese herbal mixture on the function of human osteoblastic cells hFOB 1.19 in vitro and to explore the relevant molecular mechanisms. Methods： Medicated serum was pre- pared using different doses of a kidney-reinforcing chinese herbal mixture in SD rats. hFOB 1.19 cells were treated by the medicated sera. Cellular alkaline phosphatase （ALP） activity, Osteocalcin（OC） secretion and the protein levels of osteoprotegerin （OPG） , os- teopontin （OPN） , and receptor activator of NF-KB ligand （RANKL） were examined by a ALP kit, ELISA and Western blot respec- tively. Results： The medicated serum enhanced ALP activity in hFOB 1.19 cells at the low dose （P 〈0.05） and high dose （P 〈 0.01 ）. Exposing hFOB 1.19 cells to the medicated sera for 48 h did not result in any statistical difference in OC secretion among the control and experimental groups; however, a 96 h exposure to the medicated sera promoted OC secretion（ compared with the mock con- trol, low-dose serum, P 〈0.05, and high-dose serum, P 〈0.01 ）. The medicated sera increased the expressions of OPG and OPN （ P 〈 0.05 ） , but suppressed RANKL expression（P 〈 0. 01 ） in hFOB 1.19 cells. Conclusion ： The medicated sera of the kidney- re- inforcing chinese herbal mixture may promote the differentiation and function of osteoblasts.The underlying molecular mechanism is associated with singnal regulating system of OPG/RANK/RANKL.

关 键 词：成骨细胞 补肾中药合剂 OPG RANK RANKL 种植体 

分 类 号：R285.5[医药卫生—中药学]