海洋芽胞杆菌B-9987产新型抗菌环脂肽Marinhysin A的培养基优化  

Medium Optimization for the Production of New Antifungl Cyclic Lipopeptide Marinhysin A by Bacillus Marinus B-9987

作　　者：陈杰[1] 魏鸿刚[1] 罗远婵[1] 张道敬[1] 李淑兰 田黎[3] 李元广[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]上海泽元海洋生物技术有限公司,上海200237 [3]国家海洋局第一研究所,青岛266061

出　　处：《中国生物工程杂志》2013年第1期84-89,共6页China Biotechnology

基　　金：国家"863"计划(2011AA09070402;2011AA10A202-2;2011AA10A205);国家科技支撑计划(2011BAE06B04-16)资助项目

摘　　要：Marinhysin A(MA)是由海洋芽胞杆菌B-9987产生的具有抗真菌活性的新结构环脂肽类化合物,该化合物不仅抑菌谱广而且盆栽防效良好。采用响应面分析法(Response SurfaceMethnology)对海洋芽胞杆菌B-9987产脂肽MA的培养基进行了优化。Plackett-Burman(PB)实验表明,蔗糖和酵母粉是显著影响MA产量的因素。中心组合实验(Central Composite Design)分析表明,当培养基中蔗糖和酵母粉的含量分别为42.0g/L和42.3g/L时,MA的浓度最大可达68.19mg/L,与实验值75.74mg/L相近。优化后,MA产量(250ml摇瓶发酵)较优化前的54.12mg/L提高了28.5%。用优化后培养基进行5L罐发酵,MA浓度可达182mg/L,较优化前的130mg/L提高了28.4%。The new antifungal cyclic lipopeptide Marinhysin（MA） produced by Bacillus marinus B-9987 shows the excellent activity against plant pathogens both in vitro and in vivo.In the present study,the response surface methodology（RSM） was employed to optimize the medium composition for producing MA.The results of the Plackett-Burman design showed that sucrose and yeast powder had significant effects on MA production.The central composite design was applied to further optimize the concentration of sucrose and yeast powder in the medium.The results of analysis suggested that the optimum values of the tested variables were sucrose of 42.0 g/L and yeast powder of 42.3 g/L.MA concentration reached 75.74 mg/L,which was in agreement with the predicted value of 68.19mg/L.In comparison to the production of original level（54.12mg/L） in a 250ml flask,1.28-fold increment had been obtained.By scaling u Pthe fermentation from flask to a 5L fermentor with the optimal medium,MA concentration was increased to 182mg/L,which was 1.28 times of the value（130mg/L） with the original medium.

关 键 词：Marinhysin A 海洋芽胞杆菌B-9987 培养基优化 响应面分析法 

分 类 号：Q815[生物学—生物工程]