反复胚胎植入失败患者主动免疫治疗前后封闭抗体的检测及其意义  

Clinical significance of antipaternal lymphocyte antibody before and after active immunotherapy in patients with recurrent implantation failure

作　　者：娄华[1] 王兴玲[1] 王雪梅[1] 孙丽君[1] 管一春[1] 石瑛[1] 李巍巍[1] 张霞[1] 

机构地区：[1]郑州大学第三附属医院,郑州450052

出　　处：《现代免疫学》2013年第1期52-55,共4页Current Immunology

基　　金：河南省医学科技攻关计划项目(编号2011020054)

摘　　要：探讨反复胚胎植入失败(RIF)患者封闭抗体检测的临床意义。采用荧光标记流式细胞分析技术,检测200例RIF患者、200例复发性自然流产(RSA)患者封闭抗体的水平,并选择同期正常未妊娠妇女80例作对照(对照组)。RIF组抗CD3-BE、抗CD4-BE、抗CD8-BE均显著低于对照组(P<0.05);RIF组与RSA组无统计学差异(P>0.05);RIF组主动免疫治疗后抗CD3-BE、抗CD4-BE、抗CD8-BE均显著高于主动免疫治疗前(P<0.05)。RIF可能存在封闭抗体的缺失而导致母胎免疫失调,主动免疫治疗可调节异常的细胞免疫功能。To study the clinical significance of antipaternal lymphocyte antibodies in patients with recurrent implantation failure （RIF）, 200 patients with RIF and 200 patients with recurrent spontaneous abortion（RSA） from The Third Affiliated Hospital of Zhengzhou University were enrolled in this study, in addition, 80 normal non-pregnant patients were selected as control. Antipaternal lymphocyte antibodies were detected by double-label flow cytometrie analysis. The expression frequencies of anti- CD3-BE, anti-CD4-BE and anti-CD8-BE were significantly higher in RIF group than those in the controls, but there were no significant differences between the RSA group and the RIF group. The expression frequencies of anti-CD3-BE, anti-CD4-BE anti-CD8-BE in RIF women were increased significantly after active immunotherapy. These results suggest that lack of antipat- ernal lymphocyte antibodies may be associated with RIF, and active immunotherapy might regulate the immunization function of patients with RIF.

关 键 词：反复胚胎植入失败 复发性早期流产 封闭抗体 流式细胞分析技术 主动免疫治疗 

分 类 号：R71[医药卫生—妇产科学]