兔脑炎原虫病快速诊断的临床病理学研究  

Clinicopathologic study on rapid diagnosis of encephalitozoonosis

作　　者：潘耀谦[1] 刘兴友[1] 王选年[2] 银梅[1] 唐海蓉[1] 李瑞珍[1] 刘志科[1] 孙玉倩[1] 

机构地区：[1]河南科技学院动物科学学院,河南新乡453003 [2]新乡学院生物科学技术研究中心,河南新乡453003

出　　处：《中国兽医科学》2013年第2期203-207,共5页Chinese Veterinary Science

基　　金：教育部留学回国人员科研启动基金项目(2004527);河南科技学院人才科学研究基金项目(200901)

摘　　要：为了在临床上能快速检出兔脑炎原虫,尽快诊断兔脑炎原虫病,对病兔进行了尿沉渣涂片的特殊染色、尿沉渣透射电镜的负染色、肾触片的特殊染色和肾病理组织学切片的比较观察。就尿沉渣涂片和肾触片的特殊染色而言,经光学显微镜检查,可从涂片和触片上的肾小管上皮细胞内检出兔脑炎原虫及其假囊,据此可对兔脑炎原虫病做出快速诊断。而对尿沉渣透射电镜的负染色和肾病理组织切片的检查,可与尿沉渣涂片检查结果相互印证,使临床诊断更加准确。结果表明,尿沉渣涂片和肾触片的特殊染色是临床上用于诊断兔脑炎原虫病的一种快速、准确和简便的新方法。To detect Encephalitozoon cuniculi rapidly and diagnose encephalitozoonosis as soon as possible in the clinic,the comparative observation of the specific staining urinary sediment smear of sick rabbits,the negative staining urinary sediment for transmission electron microscope,the specific staining renal-touched slides and the renal histopathologic sections were performed in this experiment.In result,Encephalitozoon cuniculi and pseudocysts in renal tubular epithelium were observed on the specific staining urinary sediment smear and renal-touched section by light microscopy.Thereby,sick rabbit with encephlitozoonosis was able to diagnose rapidly as.The examination of negative staining for transmission electron microscopy and the histopathological sections were used to corroborate the detection of urinary sediment,to make the clinic diagnosis more accuracy.The result showed that the special staining of urinary sediment smear and renal-touched slide is a rapid,accurate and simple new method for diagnosis of encephalitozoonosis in clinic.

关 键 词：兔脑炎原虫病 临床病理学 快速诊断 尿沉渣 假囊 

分 类 号：S852.729[农业科学—基础兽医学]