东北地区野生鸟类B亚群禽白血病的分子流行病学调查及env基因的序列分析  

Molecular epidemiological investigation and env gene sequence analysis of subgroup B avian leukosis virus isolated from wild birds in Northeast China

作　　者：李德龙[1,2] 刘婉思[3] 杨波[3] 高奇[1] 曾祥伟[3] 秦立廷[1] 高宏雷[1] 刘思当[2] 高玉龙[1] 王笑梅[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室,黑龙江哈尔滨150001 [2]山东农业大学动物科技学院,山东泰安271018 [3]东北林业大学野生动物资源学院,黑龙江哈尔滨150040

出　　处：《中国兽医科学》2013年第2期208-212,共5页Chinese Veterinary Science

基　　金：公益性行业(农业)科研专项(201203055);现代农业肉鸡产业技术体系建设项目(nycytx-42-G3-01);哈尔滨市科技攻关计划项目(2010AA6AN034);兽医生物技术国家重点实验室开放基金项目(SKLVBF201201)

摘　　要：为掌握我国东北地区野生鸟类感染禽白血病病毒(ALV)的情况,从黑龙江、吉林和辽宁三省采集了300份野生鸟类样品,经PCR检测,4份野生鸟类样品为ALV-B阳性,并扩增了这4份样品的env基因,对其序列进行了遗传进化分析。结果显示,4份ALV-B阳性样品的env基因与原型毒株RAV-2的同源性为99.1%～99.4%,与美国的另外2株代表毒株Schmidt-Ruppin B和Prague subgroup B的同源性为93.3%～98.4%,而与2株中国蛋鸡分离株SDAU09C2和SDAU09E3的同源性为90.2%～90.6%。以上结果表明,目前我国野生鸟类已经存在禽白血病。env基因序列分析结果表明,这4份阳性样品的env基因部分位点发生了变异,并且在多处位点存在突变。In order to analyze the situation of wild birds infected with avian leukosis virus（ALV） in Northeast China,300 samples of wild birds were collected from Heilongjiang,Jilin and Liaoning provinces.PCR showed that wild birds have been infected by ALV-B.The env genes of 4 positive samples were amplified,cloned and sequenced.In result,the env genes of the 4 samples have homology with prototype strain（RAV-2）,from 99.1% to 99.4%.But they have identity with Schmidt-Ruppin B and Prague subgroup B,from 93.3% to 98.4%.They showed identity with SDAU09C2 and SDAU09E3,from 90.2% to 90.6%.The result showed that avian leukosis virus has infected the wild birds in China.Sequences of four positive samples revealed that the part sites of the env gene varied.

关 键 词：野生鸟类 B亚群禽白血病 流行病学调查 序列分析 

分 类 号：S855.3[农业科学—临床兽医学]