针对马铃薯卷叶病毒复制酶基因负链的突变核酶的克隆及体外转录  

Cloning and Transcription In Vitro of Mutated Ribozyme Cleaving the Negative Strand RNA of Potato Leafroll Virus Replicase Gene

作　　者：杨静华[1] 乔建军[1] 张鹤龄[1] 

机构地区：[1]内蒙古大学生物学系,内蒙古呼和浩特010021

出　　处：《内蒙古大学学报（自然科学版）》2000年第3期300-303,共4页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金资助项目

摘　　要：根据特异性切割马铃薯卷叶病毒中国分离株 ( PLRV-Ch)复制酶基因负链 RNA的锤头状核酶 ,设计、合成了编码与其相应的突变核酶的 c DNA,并克隆到质粒 p GEM-4 Z中 ,经序列分析及体外转录表明得到完整的突变核酶基因重组质粒 。A mutated ribozyme gene cDNA was designed and synthesized according the hammer head structure ribozyme cleaving negative strand RNA of replicase gene of potato leafroll virus chinese isolate (PLRV Ch). The mutated ribozyme gene was cloned in plasmid pGEM 4Z named pMGR, and analyzed by sequencing. Transcription in vitro experiment revealed a mutated ribozyme RNA was obtained.

关 键 词：马铃薯卷叶病毒 复制酶基因负链 克隆 突变核酶 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治] S532.034[农业科学—植物保护]