高效液相色谱串联四级杆飞行时间质谱法同时测定舒脑欣滴丸中藁本内酯、洋川芎内酯I等6种成分含量  

Simultaneous Determination of Ligustilide,Senkyunolide I and other Six Components in Shunaoxin Dropping Pills by UPLC-Q-TOF-MS

作　　者：阎小青[1] 董林毅[2] 齐欣[3] 白钢[4] 

机构地区：[1]天津医院,天津300180 [2]天津医科大学药学院,天津300070 [3]天津中新药业集团股份有限公司第六中药厂,天津300401 [4]南开大学药学院,天津300071

出　　处：《中国药学杂志》2013年第3期216-219,共4页Chinese Pharmaceutical Journal

摘　　要：目的建立同时测定舒脑欣滴丸中尿苷、鸟苷、阿魏酸、藁本内酯、洋川芎内酯I和H含量的液相色谱-质谱联用方法(UPLC-Q-TOF)。方法采用Waters Acquity BEH C18色谱柱(2.1 mm×100 mm,1.7μm),流动相为乙腈-甲酸-水体系梯度洗脱,流速为0.4 mL.min-1,PDA检测190～400 nm扫描;进样量:2.0μL。结果尿苷、鸟苷、阿魏酸、藁本内酯、洋川芎内酯I和H分别在10.31～206.24,3.28～65.52,6.36～127.24,8.24～164.88,10.18～200.52,3.14～62.72μg.mL-1内与峰面积呈良好线性关系;精密度、重复性和稳定性良好;加样回收率在99%～101%之间。结论所建立的UPLC-Q-TOF方法简便、准确、快速、重复性好,可用于舒脑欣滴丸中尿苷、鸟苷、阿魏酸、藁本内酯、洋川芎内酯I和H的含量测定,为该药的质量控制提供依据。ABSTRACT ： OBJECTIVE To establish an LC-MS method for simultaneous determination of six compounds （ uridine, guanosine, ferulic acid, ligustilide, senkyunolide I and H in Shunaoxin dropping pills. METHODS The analysis was performed on a Waters Aequity BEH Cls column （2. 1 mm × 100 mm, 1.7 μm）with the mixture of acetonitrile-water-formic acid as the mobile phase, and the flow rate was 0. 4 mL · min^-l ; the PDA detection wavelength was 190 -400 nm; the column temperature was room temperature （30 ℃ ）. RESULTS The linear ranges for uridine, guanosine, ferulic acid, ligustilide, senkyunolide I and H were 10. 31 -206. 24, 3.28 - 65.52,6. 36 - 127.24,8.24 - 164. 88,10. 18 - 200. 52, and 3.14 - 62. 72μg · mL^-1, respectively. The average recoveries were between 99%-101% （n=6）. CONCLUSION This method is simple, accurate and reliable to determine the contents of the six compounds in Shunaoxin dropping pills for the quality control. KEY WORDS ： Shunaoxin dropping pill ; LC-MS ; UPLC ; Q-TOF-MS ; ligustilide ; senkyunolide I

关 键 词：舒脑欣滴丸 液相色谱-质谱联用 超高压液相色谱 串联四级杆飞行时间质谱 藁本内酯 洋川芎内酯I 

分 类 号：R917[医药卫生—药物分析学]