荔枝核总黄酮抗肝纤维化作用的实验研究  

A research of the effect of total flavone from Litchi chinensis Sonn on hepatic fibrosis in rats

作　　者：罗伟生[1,2] 欧士钰[1] 靳雅玲[1] 覃浩[1] 孙旭锐[1] 喻勤[1] 傅向阳[1] 

机构地区：[1]桂林医学院附属医院消化内科,广西桂林541001 [2]广西中医药大学第一附属医院消化科,南宁530001

出　　处：《重庆医学》2013年第4期373-375,378,共4页Chongqing medicine

基　　金：广西卫生厅重点课题资助项目(2010052);广西中医药药效研究重点实验室开放课题资助项目(10-046-04-K12)

摘　　要：目的观察荔枝核总黄酮(TFL)抗大鼠肝纤维化作用,并探讨其中的可能机制。方法以二甲基亚硝胺(DMN)腹腔注射制作大鼠肝纤维化模型;造模同时TFL给药组以TFL灌胃给药,秋水仙碱为阳性对照组,苏木素-伊红(HE)染色、马松染色观察大鼠肝纤维化程度,检测血清天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)水平,检测肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)含量,免疫组化检测肝组织核转录因子-κB(NF-κB)的表达。结果与模型组比较TFL给药组血清AST、ALT水平及肝组织MDA含量明显降低(P<0.05),SOD含量明显升高(P<0.05);TFL可明显抑制肝组织NF-κB的表达(P<0.05),改善大鼠肝纤维化程度(P<0.05)。结论 TFL具有显著的抗肝纤维化作用,减轻机体脂质过氧化反应及抑制NF-κB的表达可能参与了其抗肝纤维化作用的机制。Objective To observe the effects of total flavone from Litchi chinensis Sonn（TFL） on rats with hepatic fibrosis and research the possible mechanisms in it. Methods Rat hepatic fibrosis model was induced by intraperitoneal injection of dimethylnitrosamine （DMN）. Meanwhile TFL was orally given to fibrosis rats and these rats constituted the medicine group,and colchicine was used as a positive control. The degrees of fibrosis were evaluated by H. E and Masson straining,and serum trail levels of aspartate aminotransferase （AST） and alanine aminotransferase （ALT） were detected. The activity of superoxide dismutase （SOD） and content of malondialdehyde（MDA） in liver tissues were detected. The expression levels of NF-kB in liver tissue were assessed by SP immunohistochemical staining. Results Compared with the hepatic fibrosis model group, the levels of AST, ALT in serum trail and MDA in liver tissues of the TFL medicine group decreased significantly（P〈0.05） while the levels of SOD increased significantly（P〈0.05）. TFL could inhibit the expression of NF-kB in the liver tissues significantly （P〈0.05） and alleviate the hepatic fibrosis in rats （P〈0.05）. Conclusion TEL has an anti-hepatic fibrosis action and the mechanisms possibly involve effectively alleviating lipid peroxidation and inhibiting the expression of NF-kB.

关 键 词：肝硬化 脂质过氧化作用 荔枝核总黄酮 核转录因子-KB 大鼠 

分 类 号：R285.5[医药卫生—中药学]