四十里湾海洋贝类对氨基脲的生物富集特性  

Semicarbazide bioaccumulation in seashells of Sishili Bay

作　　者：于召强[1,2] 徐英江[1,3] 田秀慧[1,3] 刘义豪[1,3] 宋秀凯[1,3] 任传博[1,2] 黄会[4] 刘云[4] 张利民[1,3] 

机构地区：[1]山东省海洋水产研究所,山东烟台264006 [2]烟台山水海产有限公司,山东烟台264006 [3]山东省海洋生态修复重点实验室,山东烟台264006 [4]上海海洋大学食品学院,上海200000

出　　处：《海洋环境科学》2013年第1期39-42,共4页Marine Environmental Science

基　　金：海洋公益性行业科研专项经费资助项目(200805031;200905019和201105013);山东省水生动物营养与饲料泰山学者岗位资助项目(2006-)

摘　　要：采用超高效液相色谱-串联质谱法对四十里湾海域(设置马山寨和石沟屯两个取样点)海水及贻贝Mytilus edulis、海湾扇贝Argopecten irradias、栉孔扇贝Chlamys gloriosa和牡蛎Ostrea gigas thunberg等贝类样品中氨基脲含量分别进行检测。本方法氨基脲的线性范围为0.5μg/L～20.0μg/L,相关系数大于0.99。该研究所得的结果可为四十里湾海域贝类氨基脲污染的净化提供数据支持。Semicarbazide （SEM） in Mytilus edulis,Argopecten irradias, Chlamys gloriosa, Ostrea gigas thunberg and seawater of Sishili Bay （sampling spots were Mashanzhai and Shigoutun ） was determined using ultra performance liquid chromatogram tandem mass spectrometry （UPLC-MS/MS） in this work. UPLC and MS/MS analytical conditions were examined and optimized critically by a series of experiments. Samples were extracted and the residue was separated by ACQUITYTM UPLC BEH C18 column （ 1.7 μm,2.1 mm i. d. ×100 ram） using gradient elution separation. Mobile phase was A （methanol） and B （5 mmoL/L of ammonium acetate containing 0.1% formic acid）. Finally analyte was confirmed and quantified using MS/MS system in multiple reaction mode with triple Quad- rapole analyzer using positive polarity mode. The SEM showed good linearity in the range of 0.5 g/L to 20.0 g/L with correlation coef- ficient greater than 0.99. The study can make deeper understanding of SEM residues and distribution in different seashells. It can also provide dynamic dada and basis for guarantee health for people, continuous growth of fishing industry and government-based manage- ment and decision. The study can also provide useful information for studying decontamination of seashells that have SEM contamina- tion in Sishili Bay.

关 键 词：氨基脲 四十里湾 海水 贝类 超高效液相色谱-串联质谱法 

分 类 号：Q331[生物学—遗传学] X55[环境科学与工程—环境工程]