一株源自海洋环境多环芳烃降解菌的筛选、鉴定及适宜降解条件研究  

Study on selection,identification and optimization degrading condition for one phenanthrene train in sea

作　　者：朱生凤[1] 李红芳[2] 宫小明[3] 尤学一[1] 梁生康[4] 

机构地区：[1]天津大学环境科学与工程学院,天津300072 [2]青岛科技大学化工学院,山东青岛266042 [3]潍坊出入境检验检疫局,山东潍坊261041 [4]中国海洋大学化学化工学院,山东青岛266100

出　　处：《海洋环境科学》2013年第1期58-62,共5页Marine Environmental Science

基　　金：海洋公益性行业科研专项经费项目(201105020);国家质量监督检验检疫总局科研基金项目(2007IK154)

摘　　要：利用以菲为唯一碳源的选择性富集培养基,从天津港6号码头潮间带表层沉积物中分离到1株多环芳烃降解细菌,命名为DHD,经16SrDNA序列比对结果显示,该菌株属门多萨假单胞菌(Pseudomonas mendocina)。DHD能以多种多环芳烃化合物包括萘、菲、蒽、芘为唯一碳源和能源生长。以菲为代表,通过摇瓶培养实验考察了该菌株降解多环芳烃的适宜培养条件为:酵母浸粉为氮源,初始pH 9.0,培养温度30℃,摇床转速为200 r/min。A phenanthrene-degrading strain DHD was isolated from surface intertidal sediments in No. 6 marina of Tianjin Port by the selective enrichment culture. The strain DHD can grow on phenanthrene as a sole carbon and energy source for the growth. Variety of polycyclic aromatic hydrocarbons （PAHs） , including naphthalene, phenanthrene, anthracene and pyrenec can be degraded by the strain DHD. With the analysis of colony morphology, physiological and biochemistry experiments and 16S rDNA gene sequence, the strain DHD was identified as Pseudomonas mendocina. It was suggested that the optimization of culture condition was pH 9.0,30 and rate of 200 r/min. Meanwhile, the influence for growth of strain DHD under the glucose were determined. It shown that strain DHD could grow better in the medium eontaing the glucose as extra carbon source.

关 键 词：生物修复 多环芳烃 培养条件 菲 

分 类 号：X55[环境科学与工程—环境工程] Q939.9[生物学—微生物学]