反相高效液相色谱法同时测定飞扬草中杨梅苷和槲皮苷含量的方法探讨  

Determination of myricetrin and quercitroside in euphorbia hirta L. by RP-HPLC

作　　者：曾勇[1] 胡筱梅[2] 瞿京红[2] 

机构地区：[1]湖北省东风汽车公司茅箭医院药剂科,十堰442000 [2]湖北省东风汽车公司总医院药学部

出　　处：《国际中医中药杂志》2013年第1期46-48,共3页International Journal of Traditional Chinese Medicine

摘　　要：目的采用反相高效液相色谱法（RP．HPLC）分析飞扬草中有效成分杨梅苷和槲皮苷的含量。方法以ZORBAXSB．C18（250mm×4．6mm，5μm）色谱柱，以乙腈一0．1％磷酸溶液（21：79）作为流动相，流速为O．8ml／min，柱温：30℃；检测波长：256mm。结果杨梅苷和槲皮苷分别在O．013～0．26mg／ml、0．008～0．16mg／ml范围内有良好的线性关系，平均加样回收率分别为99．1％、98．9％，RSD分别为0．91％、1．55％。结论本法准确、简便、重复性好，可用于飞扬草中杨梅苷和槲皮苷的定量分析。Objective To establish the RP-HPLC method for the determination of myricetrin and quercitroside in Euphorbia Hirta L. Methods The ZORBAX SB-C18 （250 ram×4.6 mm, 5 μm） column was used, the mobile phase consisted of acetonitrile： 0.1% H3PO4（21 ： 79）, the flow rate was 0.8 ml/min, the column temperature was 30℃ the detecting wavelength was at 256 nm. Results The cablibration curve was linear within a range of 0.013 ～0.26 mg/ml and 0.008 ～ 0.16 mg/ml, the average recovery was 99.1%, 98.9% and the RSD was 0.91%, 1.55%, respectively. Conclusion The method is simple, repeatable and accurate, it can be applied in quantitative determination of myricetrin and quercitroside in Euphorbia Hirta L..

关 键 词：飞扬草 杨梅苷 槲皮苷 反相高效液相色谱法 

分 类 号：O65[理学—分析化学] R284[理学—化学]