氮、磷对单针藻异养生长与油脂合成的影响  

Effects of nitrogen and phosphorus on growth and lipid synthesis of Monoraphidium sp. FXY-10 under heterotrophic condition

作　　者：王琳[1] 余旭亚[1] 赵鹏[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,昆明650224

出　　处：《中国油脂》2013年第1期40-43,共4页China Oils and Fats

基　　金：云南省自然科学基金资助项目(2010CD028)

摘　　要：在异养条件下,应用Andrew方程考察了氮、磷营养盐对单针藻Monoraphidium sp.FXY-10生长的影响,并研究了氮、磷营养盐对单针藻油脂含量的影响。结果表明,磷的半饱和常数(KS,P)小于氮的半饱和常数(KS,N),说明磷对单针藻生长的影响大于氮。此外,在NaNO3质量浓度为1 g/L时,生物量产率(253.77 mg/(L·d))、油脂产率(110.36 mg/(L·d))、油脂含量(43.49%)达到最大值;在K2HPO4质量浓度为0.1 g/L时,生物量产率(323.54 mg/(L·d))、油脂产率(136.83 mg/(L·d))、油脂含量(42.29%)达到最大值。并且在氮、磷缺乏的情况下,油脂含量相对较高。The effects of nitrogen and phosphorus on growth and lipid accumulation of Monoraphidium sp. FXY - 10 under heterotrophic condition were investigated by Andrew equation. The results showed that the half saturation constant of nitrogen （ Ks,N ） was higher than that of phosphorus （ Ks ）, which indicated that the effect of phosphorus on the growth of Monoraphidium sp. FXY - 10 was greater than that of nitro- gen. Furthermore ,the biomass productivity（253.77 rag/（ L d） ） ,lipid productivity（ 110. 36 rag/（ Ld ） ） and lipid content（43.49% ）were the highest when the concentration of sodium nitrate was 1 g/L The biomass productivity（323.54 mg/（ L d） ）, lipid productivity （ 136.83 mg/（ L d） ） and lipid content （42. 29% ）were the highest when the concentration of potassium phosphate dibasic was O. 1 g/L. In the case of nitrogen and phosphorus deficiency, lipid content was relatively higher.

关 键 词：单针藻 异养条件 氮 磷 油脂含量 

分 类 号：TE667[石油与天然气工程—油气加工工程]