天南星炮制减毒机制探讨(Ⅱ)  

Investigation of Attenuating Toxicity Mechanism of Processing for Arisaema erubescens(Ⅱ)

作　　者：唐力英[1] 吴宏伟[1] 王祝举[1] 赫炎[2] 付梅红[1] 方婧[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院中医临床基础医学研究所,北京100700

出　　处：《中国实验方剂学杂志》2013年第3期1-4,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(307 72788);中国中医科学院基本科研业务费自主选题项目(ZZ20090210)

摘　　要：目的:研究天南星中特殊草酸钙针晶的组成、微观形态及毒性。方法:采用X射线衍射法及红外光谱法鉴定针晶的组成。电镜扫描法观察其微观形态,并与其他植物中的针晶进行对比研究。采用兔眼刺激试验比较针晶毒性与其质量分数的关系。结果:针晶的组成确定为草酸钙,其微观结构表面不光滑,有许多突起物(倒刺),且针体中央有一棱槽,其毒性与质量分数有明显的正相关量毒关系。结论:天南星针晶是产生刺激性毒性的主要成分,且其刺激性毒性与针晶的结构形态有关。Objective： To study on composition, micromorphology and toxicity of special calcium oxalate raphides in Arisaema erubescens. Method： X-ray diffraction and infrared spectrometry were used to identify composition of raphides. Micromorphology of raphides in A. erubescens was observed by scanning electron microscopy, and compared with that in other plants. Rabbit eye irritation experiment was used to test relationship between toxicity and concentration of raphides. Result： Composition of raphides in A. erubescens was determined to be calcium oxalate, microstructure of raphides＇ surface was not smooth, there were a number of protrusions （barbs） , and center of this needle body had a groove, its toxicity and concentration had a significant positive correlation relationship. Conclusion： Raphides in A. erubescens was main component to produce irritating toxicity, and irritating toxicity of raphides was related with its micromorphology.

关 键 词：天南星 针晶 微观结构 刺激性毒性 

分 类 号：R283.6[医药卫生—中药学]