丹参有效部位的提取工艺优选  

Optimization of Extracting Technology of Effective Components from Salvia miltiorrhiza

作　　者：金文淑[1] 张炜煜[2] 

机构地区：[1]通化万通药业股份有限公司,吉林通化134001 [2]长春中医药大学,长春130117

出　　处：《中国实验方剂学杂志》2013年第3期33-36,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省科技厅项目(20110949)

摘　　要：目的:优选丹参中脂溶性和水溶性成分的提取工艺。方法:以丹参酮ⅡA含量为指标,通过单因素试验和正交试验考察乙醇体积分数、加醇量、提取时间及次数对丹参中脂溶性成分提取工艺的影响;以丹酚酸B的含量为指标,采用L9(34)正交试验考察加水量、提取时间及次数对丹参中水溶性成分提取工艺的影响。结果:脂溶性成分最佳提取工艺为加8倍量70%乙醇提取3次,每次30 min;水溶性成分最佳提取工艺为加5倍量水提取3次,每次30 min。结论:优选的提取工艺操作简单、稳定可行,适合于工业生产,具有良好的应用前景。Objective： To optimize extraction technology of liposoluble and water soluble ingredients from Salvia miltiorrhiza. Method： With the content of tanshinone l/A as index, effect of ethanol concentration, the amount of ethanol, extraction time and frequency on extraction technology of liposoluble ingredients from S. miltiorrhiza by single factor test and orthogonal test; With the content of salvianolic acid B as index, influence of water amount, extraction time and times on water-soluble ingredients from S. miltiorrhiza by L9 （34） orthogonal test. Result： Optimal extraction technology of liposoluble ingredients was as following： extracted 3 times with 8 times the amount of 70% ethanol, 30 rain each time ; Optimum extraction technology of water-soluble constituents from S. miltiorrhiza was as following： extracted 3 times with 5 times the amount of water, 30 min each time. Conclusion： This optimized extraction technology was simple, stable and feasible, it was suitable for industrial production with a good prospect.

关 键 词：丹参 总丹参酮 总酚酸 提取工艺 正交试验 

分 类 号：R283.6[医药卫生—中药学]