Box-Behnken设计优化黄芩的浸渍-压榨提取工艺  

Optimization of Impregnating-Squeezing Extraction Technology for Scutellaria baicalensis by Box-Behnken Design

作　　者：唐梅[1] 刘剑云[1] 刘英[1] 杨金辉[1] 杨明[1,2] 

机构地区：[1]成都中医药大学,成都610075 [2]江西中医学院中药现代制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2013年第3期58-61,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优选黄芩的浸渍-压榨提取工艺。方法:采用浸渍-压榨联合提取法,选取提取时间、提取温度、溶剂倍数为考察因素,以总黄酮、黄芩苷提取率和出膏率的总评归一值(OD)为指标,通过Box-Behnken试验设计优选提取工艺。结果:最佳浸渍-压榨提取工艺为加11倍量水于60℃浸泡4 h,压榨,药渣加2倍量水洗涤后压榨,重复操作1次。总黄酮、黄芩苷提取率分别为17.630%,9.38%,出膏率42.7%,与模型预测值非常接近,说明该模型比较可靠。结论:优选的提取工艺科学、合理、可行,为中药常温提取工艺推广提供参考。Objective： To optimize impregnating-squeezing extraction technology for Scutellaria baicalensis. Method： Impregnating-squeezing joint extraction method was used, with OD of extract rate, yield of total flavonoids and baicalin as index, extraction time, extraction temperature and dosage of solvent were selected as factors, extraction technology was optimized by Box-Behnken design. Result： Optimal impregnating-squeezing extraction technology was as follows ： soaked 4 h with 11 times the amount of water at 60 ~C, squeezing extracted, add 2 times water to wash dregs, squeezing extracted, once again. Yield of total flavonoids and baicalin were 17.63% , 9.38% , respectively, extract rate was 42.7% , these datas were very close to model predictor, it showed this model was reliable. Conclusion： Optimized extraction technology was scientific, reasonable and feasible, it could provide a reference for promoting ordinary temperature extraction technology.

关 键 词：浸渍-压榨提取法 BOX-BEHNKEN设计 黄芩总黄酮 黄芩苷 出膏率 

分 类 号：R283.6[医药卫生—中药学]