HPLC同时测定四君子汤中4种指标性成分的含量  

Simultaneous Determination of Four Components in Sijunzi Tang by HPLC

作　　者：徐秋香[1] 郁红礼[1,2] 吴皓[1,3] 宫乐[1] 潘耀宗[1] 邱韵萦[1] 

机构地区：[1]南京中医药大学药学院,南京210029 [2]江苏省中药炮制重点实验室,南京210019 [3]国家教育部中药炮制规范及标准工艺研究中心,南京210019

出　　处：《中国实验方剂学杂志》2013年第3期62-65,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局中医药行业科研专项(201007010)

摘　　要：目的:建立同时测定四君子汤中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1和甘草酸含量的方法。方法:采用HPLC测定,Thermo Hypersil BDS色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.03%甲酸梯度洗脱,流速0.9 mL.min-1,柱温30℃,检测波长202,250 nm。结果:人参皂苷Rg1在1.522～12.176μg线性关系良好,平均回收率为100.86%,RSD2.34%,人参皂苷Re在1.368～10.944μg线性关系良好,平均回收率为100.57%,RSD 2.95%;人参皂苷Rb1在3.198～15.990μg线性关系良好,平均回收率97.88%,RSD 2.86%;甘草酸在5.036～40.286μg线性关系良好,平均回收率99.25%,RSD 2.97%。结论:该方法简便、准确、实用性强,可用于四君子汤中4种指标性成分的含量测定。Objective： To establish the method for determining simultaneously four components in Sijunzi Tang, ginsenosid Rgl, ginsenosid Re, ginsenosid Rb1 and glycyrrhizic acid. Method： HPLC was carried out on the Hypersil BDS column （4.6 mm × 250 mm, 5μm） with acetonitrile-0.03% phosphoric acid, gradient elution, the volume of flow 0.9 mL -min-1 , column temperature 30℃ , detection wavelength 202 nm and 250 nm. Result ： The correlation coefficients of the four components were higher than 0. 999 and the average recoveries were 100.86% , 100.57% , 97.88% , 99.25% , respectively; and RSD of the above four components were 2. 34% , 2.95%, 2.86%, 2.97%, respectively. Conclusion： The method is proved to be so easy and accurate and practical that it can be used to determine the four components in Sijunzi Tang.

关 键 词：四君子汤 人参皂苷RG1 人参皂苷RE 人参皂苷RB1 甘草酸 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]