源于同一植株陈皮挥发油成分动态变化规律研究  

Study on the Dynamic Change Law of Essential Oil Components in Citri Reticulatae Pericarpium from the Same Plant

作　　者：王坚[1] 韦正[1] 陈鸿平[1] 银玲[1] 刘荣[1] 刘友平[1] 

机构地区：[1]成都中医药大学药学院,教育部中药材标准化重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中国实验方剂学杂志》2013年第3期73-78,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072991);四川省教育厅自然科学重点项目(09ZA033)

摘　　要：目的:研究源于同一植株不同生长期陈皮挥发油成分的变化规律。方法采用水蒸气蒸馏法提取挥发油,GC-MS测定;GC-MS数据分析与AMDIS软件进行解析。结果:4批陈皮共定性、定量72个化合物,总含量分别为95.542%,92.138%,98.655%,96.201%。含量最高的2个化合物是柠檬烯与芳樟醇,分别占43.78～68.432%,9.258～15.724%。结论:4批次陈皮挥发油几乎含有相同的物质成分,各成分在含量上有一定差异。一些物质的量变规律对于揭示陈皮挥发油类成分的动态变化规律具有重要意义。Objective： To investigate the qualitative and quantitative change law of essential oil components in Citri Reticulatae Pericarpium （CRP） harvested in different time from the same plant. Method： The volatile oils were extracted by steam distillation and analyzed by GC-MS. The overlapped peaks were resolved by the programs of GC-MS data analysis and AMDIS. Result： A total of 72 compounds in the essential oils of four batches CRP were identified, which represented 95. 542% , 92. 138% , 98. 655% , and 96. 201% of the total relative content, respectively. The two compounds that have the highest content are limonene and linalool,accounting for 43.78-68. 432% and 9. 258-15. 724% respectively. Quantitative changes of some compounds present regularity. Conclusion： The essential oil of each batch CRP has almost the same chemical constituents, whereas the relative content of each constituent shows some difference. The quantitative change law of some components may have important meaning to explore the dynamic change law of volatile oil components in CRP. The combined utilization of programs of GC-MS data analysis and AMDIS can efficiently solve the difficulty in the qualitative and quantitative analysis of overlapped peaks.

关 键 词：陈皮 挥发油 同一植株 GC-MS AMDIS 

分 类 号：R284.1[医药卫生—中药学]