冰糖草总黄酮含量测定及显色方法优化  

Research on the Determination of Total Flavonoids Content and Colour Reaction System in Scoparia dulcis

作　　者：马雯芳[1,2] 邓慧连[1] 蔡毅[1] 余娇[1,3] 

机构地区：[1]广西中医药大学,南宁530001 [2]成都中医药大学,成都610075 [3]浙江省安吉县妇幼保健院药剂科,浙江安吉313300

出　　处：《中国实验方剂学杂志》2013年第3期112-115,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西壮族自治区食品药品监督管理局面上项目;广西壮族自治区壮药质量标准(第二册)(mzy2010002);广西自然科学基金创新研究团队项目(2011GXNSFF018006);广西科学研究与技术开发计划项目(桂科能10100027-1)

摘　　要：目的:建立用紫外-可见分光光度法测定冰糖草总黄酮含量的方法。方法:对显色剂的种类、浓度与用量进行考察,采用NaNO2-Al(NO3)3-NaOH显色反应体系,测定冰糖草中总黄酮含量。结果:以芦丁为对照品,测定波长为506 nm,在4.8～96.2 mg.L-1线性关系良好(r=0.999 3),平均回收率为103.71%(RSD 2.68%,n=6)。结论:该方法简便、快捷、准确,可作为冰糖草总黄酮含量测定的方法。Objective： To establish a quantitative method for the determination of total flavonoids in Scoparia dulcis by UV-VIS spectrophotometry. Method： The effects of species, concentration, and dosage of chromogenic reagent were studied, and NaNO2-A1 （NO3）3-NaOH colour reaction system was used for content determination of total flavonoids in Scoparia dulcis. Result： Using rutin as comparison sample, the detection wave length was set at 506 rim. There was good linear relationship during 4.8-96.2 mg·L-1 （r = 0. 999 3）. The average recovery rate was 103.71% （RSD 2.68% , n = 6）. Conclusion ： The method is simple, rapid, accurate and can be used for the quantitative method of S. dulcis.

关 键 词：冰糖草 总黄酮 含量测定 显色方法 优化 

分 类 号：R284.1[医药卫生—中药学]