氧化槐果碱表观油水分配系数测定  

Determination of Apparent Oil-water Partition Coefficient of Oxysophocarpine

作　　者：张蕾[1] 李婷婷[1] 陈孝健[1] 谢智勇[2] 

机构地区：[1]广州中医药大学中药学院,广州510006 [2]中山大学药学院,广州510080

出　　处：《中国实验方剂学杂志》2013年第3期130-132,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30901953);国家教育部新教师基金项目(20094425120001);国家大学生创新性实验计划项目(101055834)

摘　　要：目的:测定氧化槐果碱在正辛醇-水和正辛醇-缓冲液体系中的表观油水分配系数,为体内吸收研究提供参考。方法:以HPLC-DAD为检测手段,采用摇瓶法测定氧化槐果碱的表观油水分配系数。结果:在(37±1)°C下,氧化槐果碱在正辛醇-水体系中的表观油水分配系数为logPapp=-0.44。在pH 1.2～10.0的磷酸盐缓冲液中,测得logPapp在-0.97～-0.25;当pH<4.5时,受pH影响不显著,表现为亲水性;当pH>4.5时,logPapp随pH增大而逐渐增大。结论:该法可准确、快速测定氧化槐果碱的表观油水分配系数,并借此解析其体内动态。Objective： To determine the apparent oil-water partition coefficient of oxysophocarpine in n- octanol-water and n-octanol-phosphate buffer system, and to provide reference for the absorption study in vivo. Method： The apparent oil-water partition coefficient for oxysophocarpine was performed by using shaking flask method with HPLC-DAD as separation and detection pathway. Result： The apparent oil-water partition coefficient of oxysophocarpine was log Papp = - 0.44 in n-octanol-water system at 37 ℃. In pH 1.2-10.0 phosphate buffer solutions, log Papp was in the range of - 0.97- - 0.25. When pH 〈 4.5, there was no significant effect on it by pH. When pH 〉4.5, log Papp of oxysophocarpine showed the gradual increasing tendency with the increasing of pH. Conclusion： The methods described in the paper can be used to determine the oil-water partition coefficient of oxysophocarpine accurately, and help to predict the intracoporal process.

关 键 词：氧化槐果碱 表观油水分配系数 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]