稀土元素对冬凌草愈伤组织生长及冬凌草甲素、乙素含量的影响  

Effect of Rare-earth Elements on Callus Growth and Oridonin and Ponicidin in Rabdosia rubescens

作　　者：张艳贞[1] 董诚明[1] 苏秀红[1] 曹利华[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2013年第3期132-135,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173486);国家重大专项(2012ZX09304006)

摘　　要：目的:探讨稀土元素镧、铈对冬凌草愈伤组织生长及冬凌草甲素、乙素含量的影响。方法:冬凌草均匀愈伤组织在添加不同浓度La3+或Ce3+的培养基中培养,通过质量法测定愈伤组织鲜质量、绘制生长曲线,HPLC测定甲素、乙素含量。结果:CeCl3.7H2O浓度为1μmol.L-1时,愈伤生物量最高,是空白条件下的2.4倍。空白及对照条件下甲素含量均为0;空白条件下乙素含量最低为0.11%,随着Ce3+浓度升高而升高,当浓度为10μmol.L-1时高达0.58%;乙素随着La3+浓度升高而降低,当浓度为0.1μmol.L-1时含量高达0.44%。结论:0～10μmol.L-1的La3+及Ce3+均有利于冬凌草愈伤生物量的积累及乙素的合成,对甲素的合成无影响,将CeCl3.7H2O浓度10μmol.L-1作为最佳条件选择。Objective： To study the effect of rare-earth elements La3and Ce3 on callus growth and oridonin and ponicidin in Rabdosia rubescens. Method： The uniform callus of R. rubescens was cultivated in different concentration of La3 or Ce3 in culture medium, and the gravimetric method was used for the determination of callus fresh weight and cell growth curve ; HPLC method was used for the determination of oridonin and ponieidin content. Result： The supreme biomass of callus was 2.4 times of blank conditions when CeC13 7H20 concentration was 1 μmol·L-1. Blank and the control conditions of oridonin content were 0; ponicidin was the lowest with 0. 11% at blank condition; the ponicidin concentration increased as Ce3＋ increasing, it was up to 0.58% when Ce3＋ concentration was 10 μmol·L-1 while the ponieidin concentration decreased as La3＋ increasing, the content was 0.44% when La3＋ concentration was 0. 1μmol·L-1. Conclusion： 0-10μmol·L-1 La3＋ and Ce3 promoted R. rubescens callus biomass accumulation and ponieidin synthesis, didn＇t promote the synthesis of oridonin, the CeC13 7H20 concentration of 10μmol·L-1 was the selection of optimum conditions.

关 键 词：冬凌草 稀土元素 愈伤组织 冬凌草甲素 冬凌草乙素 

分 类 号：R282.284.1[医药卫生—中药学]