首乌方与美多芭同用对PD模型大鼠纹状体细胞外液神经化学物质的影响  

Effects of Shouwu Fang and Madopar Simultaneous on Neurochemical Substances of Striatal Extracellular Fluid in Parkinson's Disease Rats

作　　者：牛晓红[1] 王丹巧[1] 孙晓芳[2] 吴兆恩[1] 李鹏[1] 王巍[3] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]北京中医药大学基础医学院,北京100029 [3]中国中医科学院西苑医院,北京100091

出　　处：《中国实验方剂学杂志》2013年第3期155-159,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30772780)

摘　　要：目的:应用清醒自由活动大鼠脑内微透析及其相关检测技术,探讨首乌方与美多芭同时应用对帕金森病(PD)模型大鼠纹状体细胞外液神经化学物质的影响。方法:SD大鼠,随机分为对照组、模型组、美多芭组(30 mg.kg-1)、首乌方+美多芭组(含首乌方生药量18 g.kg-1+美多芭30 mg.kg-1)。单侧纹状体内注射6-羟基多巴胺造模,清醒自由活动状态下脑内纹状体微透析采样,高效液相-电化学检测样品中多巴胺(DA)及其代谢产物高香草酸(HVA)、二羟基苯乙酸(DOPAC)、羟自由基指标(2,3DHBA,2,5DHBA)浓度。结果:与美多芭组相比,首乌方+美多芭组纹状体细胞外液DA水平升高,最高达(39.15±28.70)μg.L-1,DA变化率(ΔDA)降低;羟自由基指标在观察时间内始终低于美多芭组,有9个时间点P<0.05。结论:首乌方可增加纹状体细胞外液DA递质的浓度并减少其波动;抑制羟自由基产生,对神经细胞具有保护作用。Objective： To study the synergic effects of Shouwu Fang and Madopar on striatal neurochemical substances in freely moving rats with Parkinson＇s disease （PD） by brain microdialysis and its relevant detection technology. Method： SD rats were randomly divided into control group, model group, Madopar group, Shouwu plus Madopar group. Parkinson＇s disease animal model was induced by injection of 6-hydrodopamine （6-OHDA） into striatum of SD rats. Brain microdialysis was performed and concentrations of dopamine （DA） and its metabolites homovallinic acid （metabolic protact of DA）, dihydroxyphenylacetic acid （DOPAC）, 2, 3-dihydroxy benzyl acid and 2, 5-dihydroxy benzyl acid （the index of hydroxyl radical） were detected by high performance liquid chromatography-electrochemical detector （HPLC-ED）. Result： Compared with Madopar group, Shouwu Fang plus Madopar group treatment elevated extracellular DA level in striatum with

关 键 词：脑内微透析 首乌方 左旋多巴 药动学 药效学 

分 类 号：R285.5[医药卫生—中药学]