基于红管药祛痰止咳有效组分的最佳配比研究  

Optimization of the Compositions for the Expectorant Cough Activities of Aster ageratoides

作　　者：赵海平[1] 康林之 任刚[3] 罗云[3] 张婧[3] 刘荣华[3] 何小汝[3] 杨明[3] 

机构地区：[1]江西中医学院药学院,南昌330004 [2]江西中医学院护理学院,南昌330004 [3]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2013年第3期163-166,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中药新型给药系统技术平台"十一五重大新药创制项目"(2009ZX09310-005);红管药有效部位最佳配比的药效学筛选及片剂制备(20111BBG70005-4);红管药有效部位最佳配比的药效学筛选及初步安全性评价(Y027)

摘　　要：目的:筛选红管药抗慢支的自身有效组分最佳配比。方法:①健康KM种小鼠72只,雌雄各半,随机分为正常对照组、氯化铵组(0.1 g.kg-1)、红管药总皂苷与总黄酮不同配比组(以基线等比增减设计法设定为8∶1,4∶1,2∶1,1∶1,1∶2,1∶4,1∶8,均为1.0 g.kg-1)。各组连续灌胃给药3 d,1次/d,采用气管酚红排泌法优选最佳配比。②健康KM种小鼠72只,分组及给药同①,阳性对照组给予咳必清0.04 g.kg-1。采用浓氨水引咳法优选最佳配比。结果:①在红管药总皂苷、总黄酮部位总量恒定的情况下,与空白组比较,各配比组随总皂苷所占比例增加,祛痰作用增强,反之,则减弱(P<0.05或P<0.01),但各组作用均不及氯化铵。②与空白组比较,各配比组2 min内咳嗽次数减少或咳嗽潜伏期延长均有显著性或极显著性差异,其中1∶1组(25.11±4.20)次、(60.44±10.31)s,1∶2组(22.13±7.02)次、(66.50±6.6)s均为P<0.01,且1∶2组2 min内咳嗽次数与咳必清组[(20.78±8.17)次]无显著性差异。结论:在红管药总皂苷、总黄酮部位总量恒定的情况下,2类组分不同配比的祛痰作用具有总皂苷比例依赖性;而止咳作用却不完全取决于总黄酮的比例变化,可能两者在1∶1,1∶2配比时还存在协同增效的配伍关系。有待于结合组分间化学反应、吸收与代谢相互影响以及其他药效或毒性变化深入研究。Objective： To screen the optimal compositions for the anti-chronic bronchitis activity from components of Aster ageratoides. Method： （!） Seventy-two Kuenming mice were randomly divided into the normal group, ammonium chloride group （0.1 g ·kg- 1 ） and groups of different proportions of total saponins and total flavonoids （Setting8：l, 4：1, 2：1, 1：1, 1：2, 1：4, 1：8 of total saponins and total flavonoids with increase- decrease baseline geometric proportion design method）. Every group was ig administrated at dose of 1.0 g ·kg-1 once a day for 3 days. The was taken as the indicators effect of eliminating phlegm detected by the quantity of phenolsulfonphthalein excretion for the optimization of compositions Seventy-two mice were randomly divided into groups as ditto. The positive control group was administrated carbetapentane at dose of 0.04 g .kg-1. The effect of stopping coughing against ammonia water stronger was taken as the indicators for the optimization of compositions. Result： @For the same total dosage, compared with blank group, the eliminating phlegm effects of group 8：1 （total saponins： total flavonoids） , group 4： 1, group 2： 1, group 1： 1, group 1： 2, group 1：4 and group 1：8 were increasing with the proportion of total saponins increased, and vice versa （P 〈 0.05 or P 〈 0.01 ） , but the eliminating phlegm effects of all group were not as good as ammonium chloride. @Compared with blank group, the cough frequency in 2 minutes were decreased and latency period were extended significantly for all groups （P 〈 0.05 orP〈0.01）, espeeiallyforgroupl：l （25.11±4.20） frequency （f） peratime. （2min）, （60.44± 10.31） sandgroup 1：2 （22.13±7.02） fperatime （2 min）, （66.50±6.6） s, （P〈0.01）. The effects of decreasing cough frequency in 2 minutes of group 1：2 and the carbetapentane group （20.78 ± 8.17） fper a time. （2 min） had no significant difference. Conclusion： For the same total dosage, the eliminat

关 键 词：红管药 祛痰 止咳 组分配比 

分 类 号：R285.5[医药卫生—中药学]