哮喘宁颗粒含药血清对致敏肥大细胞脱颗粒的作用  

Effect of Medicated Serum Prepared with Xiaochuanning Granules on Sensitized Mast Cell Degranulation

作　　者：尹航[1,2] 马淑骅[2] 任映[1] 王艺竹[3] 苏惠萍[1] 任传云[1] 王毅[2] 

机构地区：[1]北京中医药大学附属东直门医院,北京100700 [2]中国中医科学院医学实验中心,北京100700 [3]吉林大学基础医学院,长春130021

出　　处：《中国实验方剂学杂志》2013年第3期257-260,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院基本科研业务费项目(zz2011010;zz2011011);北京中医药大学自主选题(JYB22-JB061)

摘　　要：目的:观察哮喘宁颗粒药物含药血清对大鼠嗜碱性粒细胞株(RBL-2H3)细胞脱颗粒的影响,以探讨哮喘宁颗粒治疗支气管哮喘的疗效机制。方法:SD大鼠,均分5组,分别按哮喘宁颗粒0.4,0.2,0.1 g.kg-1、地塞米松(阳性对照)8.33×10-5g.kg-1、同体积生理盐水阴性对照,均ig,连续5 d,制备哮喘宁颗粒含药血清;以5%,10%含药血清干预RBL-2H3细胞脱颗粒进程,用钙离子成像法观察细胞脱颗粒早期细胞内钙离子的变化;用比色法检测细胞脱颗粒后β-己糖苷酶的释放量。结果:哮喘宁颗粒含药血清可以抑制Compound 48/80致敏的肥大细胞脱颗粒,在脱颗粒早期抑制细胞内钙离子的升高,并抑制β-己糖苷酶的释放。结论:哮喘宁颗粒含药血清在体外可以抑制致敏肥大细胞的脱颗粒过程,这可能是哮喘宁颗粒剂抑制哮喘I型变态反应,治疗支气管哮喘的机制之一。Objective： To explore the effect of Xiaochuanning granules on bronchial asthma. Medicated serum of Xiaochuanning granules was prepared in rat, the anti-degranulation effect on rat basophilc leukemia cell line （RBL-2H3） was observed in vitro. Method： Twenty-five adult Sprague-Dawley rats weighing between 180-200 g were randomly divided into 5 groups （n = 5 each）. Animals in control group were received normal saline orally while hexadecadrol treated rats received dose of 8.33 mg-L-1 as positive control by gavage. Xiaochuanning granules treated rats were orally received different doses of 40, 20, 10 g-L-1 respectively for 5 continuous days. Abdominal aorta blood was collected after last administration and medicated serum was separated; anti-degranulation effect was observed with 5% and 10% medicated serum respectively. The real time change of intracellular calcium was recorded by calcium imaging method and the release of β-hexosaminidase was detected by colorimetric method. Result： The medicated serum of Xiaochuanning granules could inhibit both the early and late phase of degranulation after sensitized by Compound 48/80 by the reduction intracellular calcium and β-hexosaminidase release. Conclusion： Medicated serum containing Xiaochuanning granules can inhibit sensitized degranulation inmast cells. This might be one of the mechanisms related to the curative effect of Xiaochuanning granules for treating type I allergic asthma and bronchial asthma in clinic.

关 键 词：哮喘宁颗粒剂 含药血清 肥大细胞 脱颗粒 

分 类 号：R285.5[医药卫生—中药学]