鸡枞菌多糖对免疫抑制小鼠免疫功能的影响  

Immunoloregulation effect of polysaccharide from Termitomyces albuminosus(Berk.) Heim. on immunosuppressive mice

作　　者：王思芦[1,2] 汪开毓[1,3] 赵玲[1] 陈德芳[1] 周赵英[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]西昌学院动物科学学院,四川西昌615013 [3]动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国药理学通报》2013年第1期59-63,共5页Chinese Pharmacological Bulletin

基　　金：四川省教育厅资助科研项目(No 09ZB077);教育部"长江学者和创新团队发展计划"创新团队项目(No IRT0848)

摘　　要：目的探讨鸡枞菌多糖(TAP)对环磷酰胺所致免疫抑制小鼠免疫功能的影响。方法以黄芪多糖(APS)为阳性对照,用不同剂量的TAP对免疫抑制小鼠进行腹腔注射,检测其腹腔巨噬细胞吞噬功能,免疫器官指数、血清溶血素、T淋巴细胞亚群、细胞因子、脾淋巴细胞增殖等免疫指标。结果 TAP在5～20 g.L-1浓度范围内不同程度增强巨噬细胞吞噬功能及提高免疫器官指数,且有剂量依赖性;20 g.L-1TAP可明显降低免疫低下小鼠CD4+/CD8+比值,并使IL-2、IFN-γ水平分别上升312.3%和88.1%,同时降低IL-4水平;TAP能明显提高脾淋巴细胞增殖能力。结论 TAP可提高免疫抑制小鼠体液及细胞免疫功能,且效果优于APS。Aim To investigate the immunoregulatory effect of Termitomyces albuminosus （ Berk. ） Helm. pol- ysaccharides （TAP） on immunosuppressive mice caused by cyclophosphamide （CTX）. Methods As- tragalus polysaccharides （APS） was used as positive control drug, and three different doses of TAP were in- traperitoneal injected into mice. The immunity indices such as macrophage phagocytosis, immune organ indi- ces, serum hemolysin, T lymphocyte subgroup, cell factor, and lymphocyte proliferation were tested. Re- suits The results showed that 5 -20 g · L-1TAP could dose-dependently enhance macrophage phagocy- tosis and immune organ indices. The ratio of CD4＋/CD8 ＋ was notably depressed by 20 g · L-1 TAP in im- munosuppressive mice and it turned to normal. The content of IL-2, IFN-~/ was respectively increased by 312.3% and 88. 1% ,and IL-4 was apparently degrad- ed by 20 g · L-1 TAP. The lymphocyte proliferation was notablely enhanced by TAP. Conclusion Com- pared with APS, the humoral and cellular immunity function could be more conspicuously enhanced by TAP.

关 键 词：鸡枞菌多糖 巨噬细胞吞噬功能 免疫器官指数 溶 血素 T淋巴细胞亚群 细胞因子 淋巴细胞增殖 

分 类 号：R-332[医药卫生] R284.1