外源表达持续活化型Gli2对小鼠星形胶质细胞生物学特性的影响  

The biological effects of ectopically expressing constitutively active type Gli2 on murine astrocytes

作　　者：吴文涛[1] 葛瑞民[1] 董林[1] 刘秀秀[1] 张小燕[1] 沈丽[1] 

机构地区：[1]北京大学基础医学院细胞生物学系,北京100191

出　　处：《中国药理学通报》2013年第1期98-101,共4页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)资助项目(No2011CB707700);北京大学新药创制综合大平台科研项目--药物新靶标发现与确认技术子平台项目

摘　　要：目的探讨外源表达持续活化型Gli2对小鼠星形胶质细胞生物学特性的影响。方法构建可表达持续活化型Gli2的慢病毒载体,包装慢病毒颗粒,继而感染小鼠星形胶质细胞,对其生物学特性的改变进行鉴定。结果研究发现,含有外源表达的持续活化型Gli2的小鼠星形胶质细胞在神经干细胞培养基中连续培养13 d后可获得形成细胞球的能力,且形成的细胞球与正常的小鼠神经球在形态上相近;对所形成的细胞球做免疫荧光染色,结果显示神经干细胞的特异性标志物Nestin呈阳性。结论在小鼠星形胶质细胞中外源表达持续活化型Gli2后可使其获得形成神经球样细胞球的能力。Aim To investigate the biological effects of ectopically expressing a constitutively active transcrip- tion factor Gli2 （aGli2） in murine astrocytes. Methods A lentivirus vector which could express aGli2 was constructed. Lentivirus was packaged, the murine as- trocytes were infected, and biological effects were in- dentified. Results The astrocytes with ectopie aGli2 obtained the ability to form neurospheres after 13 days＇ culture in neural stem cell medium. Structurally, the neurospheres derived from aGli2 astrocytes were indis-tinguishable from normal murine neurosphere. Immu- nofluorescence staining demonstrated that these cells expressed Nestin, the neural stem cell marker. Con- elusion Ectopic expression of constitutive active Gli2 could make the routine astrocytes obtain the ability to form neurospheres.

关 键 词：神经干细胞 中枢神经系统损伤 持续活化型 GLI2 神经球 慢病毒转染法 星形胶质细胞 NESTIN 

分 类 号：R332[医药卫生—人体生理学] R329.24[医药卫生—基础医学]