循环内皮祖细胞与冠状动脉慢血流的关系  

Reduced circulating endothelial progenitor cells is a risk factor of coronary slow flow

作　　者：李全忠[1] 韩金杰[1] 陈华[1] 莫新玲[1] 夏中华[1] 钱宗杰[1] 

机构地区：[1]桂林医学院附属医院心内科,桂林541001

出　　处：《中华心血管病杂志》2013年第1期44-47,共4页Chinese Journal of Cardiology

基　　金：国家自然科学基金地区项目（81160028,81160489）;广西科学基金（桂科回0832023）

摘　　要：目的观察冠状动脉慢血流（CSF）患者循环内皮祖细胞（EPC）数量与CSF之间的关系，探讨CSF发病的可能机制。方法选择冠状动脉造影结果正常和CSF患者各30例，采用密度梯度离心法从外周血获取单个核细胞，通过FITC标记荆豆凝集素和DiI标记的乙酰化低密度脂蛋白双染色、倒置荧光显微镜（200倍视野）鉴定EPC并对EPC进行计数。应用t检验和卡方检验比较两组患者临床资料的差异，并采用logistic回归分析法对相关因素进行分析。结果两组患者的年龄、性别、高血压、糖尿病、吸烟史所占比例及血脂水平差异均无统计学意义，CSF患者外周血EPC数量明显少于正常对照组（35．7±5．9比53．2±5．9，t=10．3，P〈0．01）。logistic回归分析显示，性别、年龄、吸烟史、高血压史、糖尿病史及血脂水平等变量都与CSF无明显相关性；而随着EPC数目增加，CSF发生概率减少（OR=0．424，95％C10．358～0．621，P〈0．01）。结论循环EPC数量减少可能是CSF独立危险因素之一，与CSF发病有关。Objective To explore if reduced number of circulating endothelial progenitor cells （EPCs） is a risk factor for patients with coronary slow flow （CSF）. Methods Thirty patients with CSF and 30 age and gender matched control subjects with normal coronary angiography were included in the study. Mononuclear cells were isolated from peripheral blood by Ficoll density gradient centrifugation and plated on fibronectin-coated culture dishes. EPCs were characterized as adherent ceils double positive for DiI-AcLDL- uptake and lectin-binding by converted fluorescence microscope （ × 200 ）. Results Smoking, diabetes mellitus, hypertension and the levels of plasma lipoprotein profile were similar between the two groups （all P 〉 0.05 ）. The number of EPCs was significantly lower in patients with CSF compared with control subjects （35.7 ± 5.9 vs. 53.2 ± 5.9,P 〈 0. 01 ）. TIMI frame counts was correlated with circulating EPCs number （ OR =0. 424,95% CI O. 358 - 0. 621 ,P 〈 0. 01 ） and not associated with gender, age, smoking, diabetes mellitus, hypertension and the levels of plasma lipoprotein profile. Conclusion Decreased circulating EPCs is an independent risk factor for CSF.

关 键 词：冠状动脉疾病 冠状血管造影术 内皮细胞 

分 类 号：R543.3[医药卫生—心血管疾病]