高分辨熔解曲线技术检测阴道念珠菌分型的研究  

Identification of Common Vaginal Candida Species by High Resolution Melting Curve Analysis

作　　者：葛玮[1,2] 骆志成[2] 魏玉平[2] 韩赛楠[2] 周雅丽[3] 

机构地区：[1]湖北省中山医院皮肤科,湖北武汉430033 [2]兰州大学院第二院皮肤科,甘肃兰州730030 [3]兰州大学第二医院中心实验室,甘肃兰州730030

出　　处：《中国皮肤性病学杂志》2013年第2期124-126,共3页The Chinese Journal of Dermatovenereology

基　　金：甘肃省自然科学基金项目(1010RJZA102);兰州大学第二医院院内基金(YJ2010-50)

摘　　要：目的探讨采用高分辨熔解曲线方法鉴定常见致病性念珠菌的可行性,为外阴阴道念珠菌病的分子流行病学及病原学研究奠定基础。方法用芽管试验、厚壁孢子试验、科玛嘉(CHRO Magar)显色培养基及API20CAUX酵母菌鉴定系统对临床致病性念珠菌进行鉴定,然后对5株标准株和80株临床分离株的基因组DNA内转录间隔区进行聚合酶链反应,对扩增产物进行高分辨熔解曲线分型。结果 5株念珠菌标准株和80株临床株聚合酶链反应扩增后,通过熔解曲线技术分析产生5种不同的峰图,即得到念珠菌5种分型。结论外阴阴道念珠菌的主要致病菌是白念珠菌(77.50%),高分辨熔解曲线方法鉴定常见的致病性念珠菌特异性强、稳定性好,是传统鉴别方法的有益补充。Objective To study the feasibility of identifing common pathogenic Candida clinically isolated from patients with vulvovaginal candidasis by high-resolution melting curve analysis （HRM） method. Methods Germ tube test, chlamydospore test, CHROMagar Candida and API20 kit system were applied to separate non-C. albicans strains from C. albicans. HRM was used to amplify internal transcribed spacer regions of ribosomal DNA from 5 type strains of 5 pathogenic Candida spp. and 80 clinical Candida isolates. Results Five types of graphic were obtainted after amplifycation from 5 Candida spp. by high-resolution melting curve a- nalysis. Conclusion Candida albicans is the most common pathogenic yeast in the vulvovaginal candidiasis （77.50%）. HRM is able to differentiate clinical Candida species and resulted in being specific, and sta- ble, which can be a good addition to traditional diagnostic approach.

关 键 词：念珠菌病 外阴阴道 念珠菌属 高分辨熔解曲线分析 

分 类 号：R711.72[医药卫生—妇产科学]