姜黄素联合人类细胞因子诱导的杀伤细胞诱导卵巢癌细胞凋亡的研究  

Pro-apoptotic effects of curcumin associated with CIK cells against ovarian carcinoma cells

作　　者：单延红[1] 崔丽峰[2] 崔满华[1] 

机构地区：[1]吉林大学第一医院妇产科,长春130041 [2]吉林大学第二医院妇产科

出　　处：《中华医学杂志》2013年第5期385-388,共4页National Medical Journal of China

摘　　要：目的观察姜黄素（Cur）联合人类细胞因子诱导的杀伤细胞（CIK）对卵巢癌SKOV3细胞诱导细胞凋亡的作用，并探讨其可能的分子机制。方法诱导脐血CIK细胞，电镜下观察Cur、CIK细胞、Cur联合CIK细胞作用后SKOV3细胞凋亡形态，RT．PCR检测各组细胞Fas、FADD、Caspase3mRNA的表达。将不同浓度的Cur分别作用于SKOV3细胞及CIK细胞，免疫印迹检测SKOV3细胞表面Fas表达及CIK细胞表面FasL的表达。MTT法检测抗FasL单克隆抗体对CIK细胞及Cur联合CIK细胞对SKOV3细胞增殖抑制率的影响。结果Cur联合CIK组与Cur组和CIK组相比，细胞凋亡的形态学改变最明显。Cur联合CIK组与Cur组和CIK组相比，SKOV3细胞Fas及Caspase3的mRNA表达水平增加（P〈0．05）；Cur组、CIK组和Cur联合CIK组对SKOV3细胞FADD的mRNA的表达无明显变化。Cur能够促进SKOV3细胞表面Fas蛋白表达，促进CIK细胞膜FasL蛋白表达。CIK组和Cur联合CIK组FasL抗体预先孵育能够明显抑制对SKOV3细胞的增殖抑制作用。结论Cur与CIK细胞联合使用诱导SKOV3细胞凋亡作用增强，其机制可能为Cur促进SKOV3细胞表面Fas表达增强，CIK细胞表面FasL表达增强，从而使SKOV3细胞内Fas表达增加，最终使Caspase3表达增高。Objective To observe the pro-apoptotic effects of Curcumin associated with CIK cells against SKOV3 cells of ovarian carcinoma and discusses the possible molecular mechanisms. Methods CIK cells were induced from umbilicus cord blood. The apoptotic morphology of SKOV3 cells was observed under electron microscope after treated with Cur, CIK cells and Cur associated with CIK cells. The levels of Fas protein on surface of ovarian cancer cells and FasL protein on surface of CIK cells after Curcumin treatment were determined by Western blot. The inhibition rates on proliferation of CIK cells and Cur associated with CIK cells after addition of FasL monoclonal antibody were detected by （thiazolyl blue tetrazolium bromide） MTY. Results The changes of apoptofic morphology in the group of Cur associated with CIK cells were most obvious compared with that in the group of Cur or CIK cells alone. Cur could promote the expression of Fas on surface of SKOV3 cells and FasL on membranes of CIK cells. The inhibition rates on proliferation in the group of CIK cells and Cur associated with CIK cells could be restrained obviously after an addition of anti- FasmAb. Conclusion The pro-apoptotic effects of SKOV3 ceils increase with the combined use of Cur and CIK ceils. The mechanism may be that Cur can promote the expression of Fas protein on cell surface of SKOV3 cells and FasL protein on cell membrane of CIK cells so as to up-regulate the expression of Fas protein in SKOV3 cells and lead ultimately to the a higher expression of Caspase3.

关 键 词：姜黄素 CIK 卵巢癌细胞 细胞凋亡 

分 类 号：R392-33[医药卫生—免疫学]