宫清方诱导T细胞亚群偏移减轻药物流产小鼠子宫出血的实验研究  

Regulating effect of Gongqing decoction on T-cell subsets to reduce the uterine bleeding of medical abortion mice

作　　者：李霞[1] 刘瑞芬[2] 王丽[1] 赵向忠[1] 周宪宾[1] 郭钰琪[1] 姜国胜[1] 姚成芳[1] 

机构地区：[1]山东省医学科学院基础医学研究所,山东省医药卫生肿瘤免疫与中药免疫重点实验室,山东省罕少见病重点实验室,卫生部生物技术药物重点实验室,国家中医药管理局免疫药理(三级)实验室,山东济南250062 [2]山东中医药大学附属医院,山东济南250011

出　　处：《山东中医药大学学报》2013年第1期42-46,共5页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:30901918);山东省科技发展计划项目(编号:2012GSF11840);山东省博士基金项目(编号:BS2009YY041);山东省自然科学基金项目(编号:ZR2010HQ066);山东省计生委项目(编号:[2009]8);山东省中医药管理局项目(编号:2009-173)

摘　　要：目的:探讨宫清方诱导T细胞亚群偏移减轻药物流产后子宫出血的作用与机制。方法:孕鼠40只,随机分为米非司酮(RU486)组[1.5 mg/(kg.d)-1]及宫清方小[77.04 mg/(kg.d)-1]、中[154.08 mg/(kg.d)-1]、大[308.16 mg/(kg.d)-1]剂量组,各10只。改良碱性正铁血红素法检测子宫出血量,流式细胞术检测母胎界面Th1(CD3+CD4+IFNγ+)、Th2(CD3+CD4+IL-4+)、Th17(CD3+CD4+IL-17A+)、Treg(CD4+CD25+Foxp3+)亚群比例,RT-PCR法检测小鼠子宫组织干扰素γ(IFNγ)、白细胞介素-4(IL-4)、白细胞介素-17A(IL-17A)、转化生长因子-β(TGF-β)mRNA转录水平。结果:宫清方作用后,药物流产小鼠子宫出血量明显减少(P<0.05),Th1、Th17亚群比例(P<0.05)及Th1/Th2、Th17/Treg比值升高(P<0.05),Th2、Treg亚群比例降低(P<0.05),IFNγ、IL-17A mRNA表达升高,IL-4、TGF-βmRNA表达降低。结论:宫清方能优势诱导药物流产小鼠T细胞向Th1、Th17亚群偏移,进而有效减少药物流产后子宫出血。Objectives：To explore the mechanism underlying the bleeding reducing effect of Gongqing decoction via regulating T-cell subsets. Methods ：Total 40 pregnant mice were assigned into mifepristone （RU486） group （ 1.5 mg/（kg·d）-1）, Gongqing de- coction low-dose [77.04 mg/（kg·d）-1] ,middle-dose [ 154.08 mg/（kg·d）-1] and high-dose [308.16 mg/（kg·d）-1] groups randomly （n=10 for each）. The volume of uterine hemorrhage was measured by the method of alkaline hematin photometric. The percentages of Thl subset（CD3＋CD4＋IFN-γ）,Th2 subset（CD3＋CD4＋IL-4＋）,Thl7 subset（CD3＋CD4＋IL-17A＋） and Treg subset（CD4＋CD25＋Foxp3＋） in mice uterine tissue were detected by flow cytometry. The mRNA expressions of IFNγ,IL-4,IL-17A,TGF-β were analyzed us- ing RT-PCR. Results：Compared to RU486 group,the uterine bleeding volume reduced significantly in Gongqing decoction groups, The percentages of Thl and Thl7 cells increased while the percentages of Th2 and Treg cells decreased after Gongqing decoction administration（P〈0.05）. Additionally,Gongqing decoction up-regulated IFN-γ and IL-17A mRNA expression and down-regulated IL-4 and TGF-β mRNA expression in a dose-depended manner（P〈0.05）. Conclusion：Gongqing decoction reduced the uterine bleeding volume of RU486-induced abortion mice efficiently via promoting Thl and Th17 skews.

关 键 词：药物流产 子宫出血 宫清方 T细胞亚群 小鼠 

分 类 号：R285.5[医药卫生—中药学]