PTSD大鼠海马RGMa的表达变化  

Changes of RGMa expression in the hippocampus of rats with posttraumatic stress disorder

作　　者：谢菊华[1] 韩芳[1] 石玉秀[1] 

机构地区：[1]中国医科大学基础医学院组织学与胚胎学教研室,辽宁沈阳110001

出　　处：《解剖科学进展》2013年第1期39-41,46,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金资助项目(No.31140060)

摘　　要：目的观察RGMa(repulsive guidancemoleculea)在创伤后应激障碍(posttraumatic stress disorder,PTSD)大鼠海马神经元的表达变化。方法建立连续单一刺激(single prolonged stress,SPS)的Wistar大鼠PTSD模型,随机分为SPS刺激后第7天组(SPS7d)、第14天组(SPS14d)及正常对照组,采用免疫组织化学、Western Blot方法检测各组海马神经元RGMa的表达变化。结果免疫组织化学及Western Blot结果均显示SPS7d组海马神经元RGMa表达水平较正常对照组增加(P<0.01),第14天组降低但仍高于正常对照组(P<0.01)。结论 PTSD模型大鼠海马RGMa蛋白水平变化可能是海马神经元遭遇巨大应激源后修复异常并最终导致神经元凋亡、海马体积缩小的重要分子机制之一。Objective To observe the changes of RGMa（repulsive guidance molecule a）expression in the hippocampus of rats with posttraumatic stress disorder（PTSD）. Methods 45 Wistar rats were randomly assigned to three groups： normal control, single-prolonged stress （SPS）groups for 7d and 14d. The changes of RGMa expression in the hippocampus of rats were examined by immunohistochemistry and Western blot. Results The expression levels of RGMa in the hippocampus were increased markedly in SPS groups than in normal control group（P〈0.01）, reached the highest at SPS7d （P〈0.01） by Western blotting and immunohistochemistry. Conclusion RGMa may be involved in the molecular mechanism of neuronal apoptosis and reduced hippocampal volume of PTSD rat.

关 键 词：创伤后应激障碍 海马 RGMa WISTAR大鼠 

分 类 号：R338.26[医药卫生—人体生理学]