超顺磁氧化铁标记对干细胞转铁蛋白受体和铁蛋白轻链基因及蛋白表达的影响  

Expression of transferrin receptor and Ferritin light chain genes in the stem cells with super paramagnetic iron oxide

作　　者：贾乾君[1] 梁长虹[1] 刘再毅[1] 颜丽芬[1] 王广谊[1] 

机构地区：[1]广东省医学科学院广东省人民医院放射科,广东广州510510

出　　处：《生物医学工程与临床》2013年第1期72-76,共5页Biomedical Engineering and Clinical Medicine

基　　金：国家自然科学基金资助项目(30970798)

摘　　要：目的研究超顺磁氧化铁(SPIO)标记对大鼠脂肪干细胞(ADSCs)转铁蛋白受体(TfR)和铁蛋白轻链(Fn-L)基因及蛋白表达的影响。方法实验通过医院伦理委员会的批准。标记组(实验组)采用多聚赖氨酸(PLL,终质量浓度为1.5μg/mL)介导SPIO(终质量浓度为50μg/mL)标记ADSCs。在2、4、8、16、24、96、168、336、504、672 h,分别用实时荧光定量聚合酶链反应(RT-PCR)和WesternBlot实验定量检测实验组和未标记组(对照组)TfR和Fn-L基因及蛋白表达水平。结果 PLL-SPIO标记ADSCs后,实验组Fn-L mRNA(2、4、8、16、24、96、168、336 h)及蛋白(16、72、96、168 h)(P均<0.05)表达水平会暂时性升高,至标记后一定时间,Fn-L mRNA(504、672h)及蛋白(336、504、672h)表达水平两组间差异无统计学意义(P均>0.05);实验组TfR mRNA(2、4、8、16、72、96、168、336 h)及蛋白(24、96 h)(P均<0.05)表达水平会暂时性减低,至标记后一定时间,TfRmRNA(504、672 h)及蛋白(168、336、504、672 h)表达水平两组间差异无统计学意义(P均>0.05)。结论在一定浓度内,PLL-SPIO标记ADSCs,对Fn-L和TfR基因及蛋白表达仅产生暂时性影响;从而为细胞内标记过程的安全性提供了实验依据。Objective To investigate the influence of superparamagnetic iron oxide（SPIO） particles on the gene and protein expression of transferrin receptor（TfR） and ferritin light chain（Fn-L） in adipose-derived stem cells（ADSCs）.Methods The experimental group were ADSCs labeled with SPIO（final concentration of 50 μg/mL） in combination with poly-L-lysine（PLL,final concentration of 1.5 μg/mL）.The gene and protein expression of TfR and Fn-L in SPIO-PLL labeled and unlabeled ADSCs were evaluated by reverse transcription polymerase chain reaction（RT-PCR） and Western blot at 2-hour,4-hour,8-hour,16-hour,24-hour,96-hour,168-hour,336-hour,504-hour and 672-hour after labeling.Results Intracellular magnetic labeling of ADSCs resulted in increased transient expression of Fn-L mRNA（2-hour,4-hour,8-hour,16-hour,24-hour,96-hour,168-hour and 336-hour） and protein（16-hour,72-hour,96-hour and 168-hour）（P 0.05）.However,there was no significant differences between the 2 groups in terms of Fn-L mRNA expression starting after 504-hour and Fn-L protein after 336-hour（P 0.05）.While TfR mRNA（at 2-,4-,8-,16-,72-,96-,168-and 336-hours after labeling） and protein（at 24-and 96-hour） were reduced temporarily（P 0.05）,there was no significant differences between 2 groups in terms of Fn-L mRNA expression starting from 504-hour after labeling and Fn-L protein from 168-hour after labeling（P 0.05）.Conclusion It is demonstrated that within a certain concentration,SPIO labeling has only temporary influence on the expression of Fn-L and TfR in ADSCs,which validates the safety of the procedure for cellular labeling.

关 键 词：超顺磁氧化铁 脂肪干细胞 标记 转铁蛋白受体 铁蛋白轻链 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]