模式生物斑马鱼对川续断皂苷VI的代谢研究  

Metabolism study of asperosaponin VI by using zebrafish

作　　者：韦英杰[1] 薛小露[1] 刘炜[1] 王长梅[1] 詹扬[1] 贾晓斌[1] 

机构地区：[1]江苏省中医药研究院,国家中医药管理局中药口服释药系统重点研究室,江苏南京210028

出　　处：《药学学报》2013年第2期281-285,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30973978);江苏省中医药领军人才专项(2006);江苏省自然科学基金资助项目(BK2011866)

摘　　要：首次采用模式生物斑马鱼研究续断中主要活性成分川续断皂苷VI的代谢,探索斑马鱼用于微量中药皂苷类成分代谢的可行性及合理性。采用高效液相色谱电喷雾质谱检测,Zorbax Extend C18(150 mm×4.6 mm,5μm)色谱柱,0.05%甲酸乙腈0.05%甲酸水梯度洗脱,正、负离子模式同时检测,根据准分子离子峰获得化合物分子量信息,通过与文献数据或对照品对照,鉴定川续断皂苷VI经斑马鱼暴露24 h后的药液及鱼体中的代谢产物。结果川续断皂苷VI经斑马鱼作用后,产生了脱去1分子、2分子及全部糖基的5种脱糖基代谢产物和1个羟基化产物,这与川续断皂苷VI在大鼠体内的代谢机制一致,提示斑马鱼对川续断皂苷VI的代谢具合理性,更具有化合物用量少,成本低、简单、高效的优势,特别是能体现在体代谢的综合结果,有望成为快速预测少量中药成分代谢、补充现有模型不足的全新的、模式生物代谢模型。Model organism zebrafish was used to study metabolism of asperosaponin VI from Dipsacus asper Wall. ex Henry for the first time. Metabolic components of asperosaponin VI after exposing to zebrafish for 24 h were identified by high performance liquid chromatography ？ electrospray mass spectrometry （HPLC- ESI-MS）, the separation was performed with a Zorbax C18 column using a binary gradient elution of 0.05% formic acetonitrile ？ 0.05% formic acid water. The quasi-molecular ions of compounds in both negative and positive mode were observed for molecule mass information, and the potential structures were identified by attentive study on the deglycosylation metabolites and one hydroxylation metabolite of asperosaponin VI. The results were highly in consistent with metabolism of asperosaponin VI in rat. It can be concluded that zebrafish model can wonderfully imitate current metabolic model with advantages of small amount of lower cost, far less amount compound, higher efficiency and more simple, and can reflect integrated metabolism results of in vivo method. Zebrafish metabolic model may become a novel organism model for quick predication on metabolism of even mircoamount compound, which can enrich the available models greatly.

关 键 词：斑马鱼 川续断皂苷VI HPLC-ESI MS 

分 类 号：R917[医药卫生—药物分析学]