基因枪介导擎天凤梨遗传转化体系的建立  

Establishment of Particle Bombardment-mediated Genetic Transformation System of Guzmania Bromeliad

作　　者：沈晓岚[1] 王炜勇[1] 毛碧增[2] 俞少华[1] 田丹青[1] 俞信英[1] 

机构地区：[1]浙江省农业科学院花卉研究开发中心,杭州311202 [2]浙江大学生物所,杭州310029

出　　处：《分子植物育种》2013年第1期77-84,共8页Molecular Plant Breeding

基　　金：浙江省重大科技专项重点项目(2009C12095);浙江省科技厅公益性研究项目(2012C22085)共同资助

摘　　要：擎天凤梨的愈伤组织获取较为困难、而且愈伤分化缓慢且极易死亡,使用农杆菌介导转基因面临着受体难以获得、侵染后愈伤死亡率高、转化时间长以及成本高等问题。使用基因枪介导的转基因方法相对来说更为直接快速,但也面临转化效率不高、插入基因表达定位不清等问题。本研究以擎天凤梨组培苗的茎尖生长组织为材料,以基因枪介导结合GFP荧光蛋白基因,对转化中的主要影响因子进行研究,建立并优化了遗传转化体系,获得了转化再生植株,并利用GFP荧光蛋白基因的荧光特性,分析确定了外源基因的表达定位,为进一步利用分子育种手段培育擎天凤梨新品种打下基础。It is known to hardly obtain the callus of Guzmania Bromeliad, and that the plant regenerates slowly with the high rate mortality, which make Agrobacterium-mediated genetic transformation of Guzmania Bromeliad more difficulties that face difficulty to obtaining explant, high mortality after infection, transformation time con- sumption and high costs. Particle bombardment-mediated genetic transformation is considered a more direct and quick way, but the problems of low transgenic efficiency and the unclear gene inserting sites exist. In this research we used top stem shoot of cultured seedling of Guzmania Bromeliad as material to study the main factors affecting genetic transformation, in order to establish and optimize the genetic transformation system of Guzmania Bro- meliad as well as obtain transgenic regenerated plant and detect and analyze the exogenous gene positioning and expression by using the Green fluorescent protein （GFP）. The achievement of this research may lay the foundation to breed new Guzmania Bromeliad varieties by using molecular breeding approach.

关 键 词：擎天凤梨 基因枪 遗传转化体系 GFP荧光蛋白基因 

分 类 号：S668.3[农业科学—果树学]