红肉猕猴桃新品系SF-HY-0201遗传特性  

Genetic Characteristics of a New Red-flesh Kiwi Fruit Line SF-HY-0201

作　　者：谢玥[1] 潘美玲[2] 庄启国[1] 王丽华[1] 郑晓琴[1] 廖明安[2] 李明章[1] 

机构地区：[1]四川省自然资源科学研究院,成都610015 [2]四川农业大学园艺学院,雅安625014

出　　处：《分子植物育种》2013年第1期99-106,共8页Molecular Plant Breeding

基　　金：国家科技部项目(2009DFA30870);四川省国际科技合作与交流研究计划项目;四川省省属科研机构基本科研业务费项目共同资助

摘　　要：以红肉猕猴桃品种＂红阳＂为对照,通过生物学特性调查、体细胞染色体数目的观察和ISSR标记分析,对红肉猕猴桃新品系SF-HY-0201形态水平、细胞水平和分子水平的遗传特性进行了检测和比较。结果表明,SF-HY-0201和红阳的花、叶、果等生物学特性均存在显著差异;SF-HY-0201和红阳的染色体均为2n=2x=58,都是二倍体;7对ISSR引物共扩增出56条带,其中多态性条带27条,达48.21%,各引物扩增的多态性条带数在1~8条之间,能揭示红阳与SF-HY-0201间的遗传差异,为今后猕猴桃育种和杂交亲本的选择提供依据。Genetic variation at the morphological, cellular and molecular levels between a red-flesh, kiwifruit cultivar Hongyang and a new red-flesh kiwifruit line SF-HY-0201 were evaluated through investigating biological characteristics and somatic chromosome number as well as identification by ISSR marker. The results showed that SF-HY-0201 and Hongyang in the biological characters of flower, leaf and fruit existed significant difference. SF-HY-0201 and Hongyang were diploids with 2n=2x=58. Seven of ISSR primers selected from 100 candidates were applied to carry out PCR amplification that generated 56 bands, 27 of them accounting for 48.21% were polymorphic. The number of polymorphic bands generated by each primer ranged from 1 to 8, which could reveal the genetic differences between Hongyang and SF-HY-0201. In conclusion, we thought this research would provide the theoretical foundation for genetic improvement and selection of cross-bred parents in kiwifruit breeding program.

关 键 词：猕猴桃 生物学特性 染色体数目 ISSR 

分 类 号：S663.4[农业科学—果树学]