血清抗精子抗体阳性对青春期雄性大鼠睾丸组织及睾丸生殖细胞中Fas/Fas-L凋亡途径的影响  

Effects of serum anti-sperm antibodies positive on the Fas/Fas-L apoptosis pathway in the testis tissue and testicular germ cells of pubertal male rats

作　　者：智二磊[1] 徐计秀[1] 王璟琦[1] 徐宁[1] 吉鹏[1] 吴卓[1] 

机构地区：[1]山西医科大学第一附属医院泌尿外科,山西太原030001

出　　处：《中华男科学杂志》2013年第2期111-115,共5页National Journal of Andrology

摘　　要：目的:通过人工免疫雄性大鼠获得抗精子抗体(AsAb)介导的血清AsAb阳性的免疫性不育大鼠动物模型,观察AsAb对青春期大鼠睾丸组织及睾丸生殖细胞中Fas/Fas-L凋亡途径的影响。方法:5周龄(青春期)雄性Wistar大鼠30只,其中10只处死,取精子制备精子悬液免疫大鼠,余20只动物随机分为实验组(10只)和对照组(10只),4周后摘取睾丸。光镜观察睾丸组织改变,免疫组化法检测Fas、Fas-L和Caspase-3蛋白的表达。结果:实验组睾丸组织切片呈凋亡样改变,实验组Fas、Fas-L及Caspase-3蛋白的OD值(176.97±4.58,187.52±7.76,157.65±7.38)较对照组(161.87±5.37,150.27±8.65,120.37±6.76)显著增高(P<0.01)。结论:同种精子免疫大鼠,可成功制作AsAb介导的免疫性不育模型;AsAb影响雄性大鼠的生育力,其机制可能与Fas/Fas-L凋亡途径中Fas、Fas-L和Caspase-3蛋白的表达升高有关。Objective： To establish a rat model of anti-sperm antibody（AsAb）-mediated immune infertility,and investigate the effects of serum AsAb positive on the Fas/Fas-L apoptosis pathway in testis tissue and testicular germ cells of pubertal male rats.Methods： Thirty 5-week-old Wistar male rats were included in this study,10 killed for preparation of sperm suspension,10 as normal controls,and the other 10 made models of AsAb-positive immune infertility（experimental group）.Four weeks after modeling,the testes of the rats were harvested for observation of the changes in the testis tissue under the light microscope and detection of the expressions of Fas,Fas-L and Caspase-3 proteins by immunohistochemistry.Results： Compared with the control group,the experimental group showed obvious apoptotic changes in the testis tissue and remarkably increased expressions（OD value） of Fas（161.87±5.37 vs 176.97±4.58）,Fas-L（150.27±8.65 vs 187.52±7.76） and Caspase-3（120.37±6.76 vs 157.65±7.38）（P0.01）.Conclusion： Serum AsAb affected the infertility of pubertal male rats,and its mechanisms might be associated with up-regulated expression of Fas,Fas-L and Caspase-3 proteins in the Fas/Fas-L apoptotic pathway.

关 键 词：抗精子抗体 生殖细胞 FAS Fas—L途径 青春期大鼠 

分 类 号：R392[医药卫生—免疫学]