大鼠骶丛撕脱伤后脊髓前角退变规律  

Degeneration law of anterior horn of spinal cord in rats with sacral plexus avulsion

作　　者：江曦 陈爱民 杨迪 朱磊 李永川 张志凌 鹿楠 李菁 沈笛 朱清华 林浩东 赵良瑜 叶添文 

机构地区：[1]第二军医大学第二附属医院创伤骨科,上海200003

出　　处：《中华创伤杂志》2013年第2期156-159,共4页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（30973054,81171161）

摘　　要：目的研究大鼠单侧骶丛撕脱伤后脊髓前角运动细胞元的退变规律。方法选用体重200—250g成年sD大鼠60只，建立单侧骶丛撕脱伤模型，分别于损伤后2，4，6，8，10，12周取大鼠脊髓L4～6 节段切片，行HE染色检测脊髓前角运动神经元的存活率；TUNNEL染色检测脊髓前角运动神经元的凋亡指数。结果撕脱后运动神经元的存活率：2周（92．1±4．7）％，4周（83．6±3．7）％，6周（43．6±4．2）％，8周（32．1±3．5）％，10周（18．4±3．7）％，12周（12．1±3．3）％。损伤侧脊髓前角运动神经元存活率随着损伤时间延长逐渐降低；而凋亡指数随着损伤时间延长逐渐升高，6周时升高比较明显。结论大鼠骶丛撕脱损伤术后6周可作为脊髓前角运动神经元变化的时间节点，神经修复术宜在此前完成。Objective To evaluate the degenerative regularity of motoneurons in anterior horn of spinal cord after unilateral sacral plexus avulsion in rats. Methods Sixty adult SD rats weighing 200- 300 g were chosen to establish models of unilateral sacral plexus avulsion. Spinal cord segments of L4-6 were removed and sectioned at postinjury 2, 4, 6, 8, 10, 12 weeks respectively to detect survival rate of motoneurons in anterior horn of spinal cord using HE staining and to detect their apoptotic index using TUNNEL staining. Results Survival rates of motoneurons at weeks 2, 4, 6, 8, 10, 12 after avulsion were （92.1±4.7）%, （83.6±3.7）%, （43.6±4.2）%, （32.1 ±3.5）%, （18.4±3.7）%, and （ 12.1 ± 3.3 ） % , respectively. Consequently, survival rate of motoneurons in anterior horn of spinal cord was decreased gradually as the expansion of injury time, but their apoptotic index presented a gradual increase with time, especially at 6 weeks after injury. Conclusion Six weeks after sacral plexus avulsion in rats may be taken as the time node of changes of motoneurons in anterior horn of spinal cord and nerve repair should be completed in advance of the time node.

关 键 词：腰胝丛 脊髓 前角细胞 大鼠 

分 类 号：R683[医药卫生—骨科学]