角膜修复用改性明胶交联膜的生物相容性评价  

Evaluation of Biocompatibility of Modified Gelatin Composite Membranes for Corneal Regeneration

作　　者：龙玉宇[1,2] 任力[1,2] 王家鸣[1,2] 陈敏[3] 刘杨[1,2] 刘炳乾[3] 王迎军[1,2] 葛坚[3] 

机构地区：[1]华南理工大学材料科学与工程学院,广州510641 [2]华南理工大学国家人体组织功能重建工程技术研究中心,广州510641 [3]中山大学中山眼科中心,广州510060

出　　处：《生物医学工程学杂志》2013年第1期170-175,共6页Journal of Biomedical Engineering

基　　金："十二五"国家科技支撑计划项目资助(2012BAI17B02);国家自然科学基金资助项目(50803018;51273072);国家863计划项目资助(2011AA030105);广东省重大科技专项项目资助(2010A080407008;2012A080203010);广东省教育部产学研结合项目资助(2010B090400416);眼科学国家重点实验室创新基金资助项目(2011C05)

摘　　要：本文对自制的改性壳聚糖-明胶交联膜(MC-Gel)与壳聚糖-明胶交联膜(CS-Gel)的理化性能、生物相容性进行测定与评价,探讨其用于角膜组织修复与重建的可行性。采用紫外分光光度计测定了交联膜的透光率及渗透性,结果显示两组交联膜的透光率超过90%,且对葡萄糖、色氨酸与NaCl的渗透率均接近或优于人角膜的渗透率,可满足角膜修复对透光率及营养物质透过的要求。同时,采用MTT法测定了人角膜上皮细胞(HCEC)在两组膜上的生长活性,并评价了两组交联膜在兔眼角膜内的组织相容性。实验结果表明,MC-Gel能较好地支持HCEC在其上增殖,且植入兔眼角膜后无明显炎症反应,术后4个月材料全部降解。因此,该交联膜具有良好的理化性能与眼组织相容性,有望成为一种良好的角膜修复材料。In order to investigate the feasibility of the modified chitosan-gelatin crosslinked membrane （MC-Gel） and chitosan-gelatin crosslinked membrane （CS-Gel） to be a potential biomaterial for corneal regeneration, we evaluated their physicochemical properties and intraocular biocompatibility in this study. White light transmission and permea- bility of these membranes were detected. Results showed that white light transmission of both membranes was above 90% at 500 nm, which was similar to that of human cornea. The glucose, tryptophan and NaC1 permeability of MC- Gel membrane and CS-Gel membrane was better than or similar to those of human cornea. The methylthiazol tetrazo- lium （MTT） assay was used to assess cell viability and proliferation. Also, interlamellar corneal transplantation was carried out to evaluate ophthalmic biocompatibility of MC-Gel membrane and CS-Gel membrane. Results indicated that MC-Gel membranes could support the proliferation of HCEC and displayed good intraocular biocompatibility when implanted into rabbits. No severe inflammatory reaction occurred after transplantation and the implanted MC- Gel membrane degraded completely 16 weeks post-operation. Due to its good physicochemical properties and biocom- patibility, MC-Gel membrane could be a promising candidate material for corneal regeneration.

关 键 词：壳聚糖 明胶 渗透性 生物相容性 层间角膜移植 

分 类 号：R318.1[医药卫生—生物医学工程]