长期艾烟干预对Wistar大鼠外周血T淋巴细胞亚群及CD4^+CD25^+Treg的影响  

Effects of long-term intervention of moxa smoke on T lymphocyte subsets and CD4^+CD25^+Treg in peripheral blood of Wistar rats

作　　者：刘平[1] 潘秀颉[2] 韩丽[1] 杨佳[1] 胡海[1] 蔡虹[1] 李若曦[2] 朱茂祥[2] 杨陟华[2] 赵百孝[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]北京军事医学科学院放射与辐射医学研究所

出　　处：《中国针灸》2013年第2期145-148,共4页Chinese Acupuncture & Moxibustion

基　　金：国家重点基础研究发展计划(973)项目:2009CB522906;国家自然基金面上项目:81072862;教育部博士点课题项目:20090013110004

摘　　要：目的:探讨长期艾烟干预对细胞免疫的影响。方法:32只Wistar大鼠随机分为空白组、低浓度组、中浓度组和高浓度组,每组8只。除空白组外,其余各组每日在相应浓度的艾烟环境中暴露20min,6个月后用流式细胞仪检测各组大鼠外周血中的T淋巴细胞亚群及CD4+CD25+调节性T细胞(CD4+CD25+Treg)占CD4+细胞的比例。结果:与空白组相比,艾烟干预各组CD3+CD4+、CD3+CD8+T细胞比例及CD3+CD4+/CD3+CD8+比值差异无统计学意义(P>0.05),但CD4+CD25+Treg占CD4+细胞的比例均显著降低(P<0.05),各项指标在艾烟干预各组间均差异无统计学意义(P>0.05)。结论:长期艾烟干预对CD3+CD4+、CD3+CD8+T细胞及CD3+CD4+/CD3+CD8+无影响,但可下调大鼠外周血中CD4+CD25+Treg在CD4+T细胞中的比例,艾烟预处理可能通过该途径发挥对机体的免疫调节作用。Objective To investigate the cellular immune regulation of the long-term intervention of moxa smoke. Methods Thirty-two Wistar rats were randomly divided into a blank group, a low concentration group, a medium concentration group and a high concentration group, 8 cases in each group. In addition to the blank group, rats in the other groups were exposed to the corresponding concentration moxa smoke for 20 min every day, the T lymphocyte subsets and proportion of the CD4＋ CD25＋ Treg in CD4＋ T cells in peripheral blood were tested by flow cytometry after 6 months. Results Compared with the blank group, the proportions of CD3＋ CD4＋ .CD3＋ CD8＋ T ceils and CD3＋ CD4＋/CD3＋ CD8＋ in the other 3 moxa smoke groups were not significantly different （P〉 0.05）, while the proportions of the CD4＋ CD25＋ Treg in CD4＋ T ceils were significantly lower （P^0.05）, but no statistically significant differences among those 3 moxa smoke intervention groups （P^0.05）. Conclusion Long- term moxa smoke intervention has no significant effect on the proportions of CD3＋ CD4＋ , CD3＋ CD8＋ T cells and CD3＋ CD4＋/CD3＋ CD8＋ , but it can decrease the proportions of the CD4＋ CD25＋ Treg in CD4＋ T cells in periph- eral blood of rats, The way produced by pretreatment with moxa smoke may play immunomodulatory effect.

关 键 词：艾烟 长期干预 T淋巴细胞亚群 CD4+CD25+TREG 大鼠 

分 类 号：R245.9[医药卫生—针灸推拿学]