材料表面性质对微生物附着行为的影响  

Influence of different material surfaces on microbial adhesion

作　　者：苏艳[1] 段继周[2] 段东霞 蔺存国 张杰[2] 侯保荣[2] 杨东方[1] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国科学院海洋研究所,山东青岛266071 [3]青岛双瑞防腐防污工程有限公司,山东青岛266101

出　　处：《海洋科学》2012年第12期56-63,共8页Marine Sciences

基　　金：中国科学院重要方向性项目(No.KZCX2-EW-205);国家自然科学基金项目(40976046)

摘　　要：利用荧光显微镜计数法,研究了微生物在4种不同材料表面的初始附着行为,对微生物附着进行了定量描述,初步获得以下结果:(1)不同材料表面,细菌的附着量呈现如下差异:316L不锈钢>PVC>玻璃,在有机硅材料(道康宁T2)表面的附着变动性很大;(2)同种材料表面,不同细菌的附着量也不同,表现为:Shewanella oneidensis MR-1菌株和Pseudomonas aeruginosa PAO1菌株均大于Escherichia coli JM109菌株。并由此对其附着差异和影响因素进行了讨论。Biofouling has great impact on marine vessels and engineering facilities, resulting in development of marine economy. It is very important to study the regular pattern of biofouling huge losses to the and develop new antifouling materials for controlling biofouling. Here we studied the initial attachment behavior of bacteria on 4 different materials surface with fluorescence microscopy. The results show that： （1） The attachment ability of bac- teria on different kinds of materials is different with an order： 316L stainless steel 〉 PVC 〉 Glass. It is worth men- tioning that the bacteria attachment ability on silicone material （Dow CorningT2） varied widely. （2） Different kinds of bacteria also show different attachment ability on the same material surface. It is easier for S.Oneidensis MR-1 and P.aeruginosa PAO1 strain to adhere on materials than E.lico JM09 strain. In this paper, the different attachment ability of bacteria and the influence factors were discussed

关 键 词：微生物附着 荧光显微镜计数 有机硅 不锈钢 玻璃 聚氯乙烯 

分 类 号：Q939.98[生物学—微生物学]