翘嘴鲌(Culter alburnus)TMEM-57基因的克隆、组织表达和单核苷酸多态性分析  

CLONING,TISSUE EXPRESSION AND SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS OF CULTER ALBURNUS TMEM-57

作　　者：祁鹏志[1] 郭宝英[2] 谢从新[1] 吴常文[2] 陆诗敏[1] 段友健[1] 

机构地区：[1]华中农业大学水产学院,武汉430070 [2]浙江海洋学院国家海洋设施养殖工程技术研究中心,舟山316004

出　　处：《海洋与湖沼》2012年第6期1196-1201,共6页Oceanologia Et Limnologia Sinica

基　　金：国家科技支撑计划,2012BAD25B06号

摘　　要：采用RT-PCR和快速扩增cDNA末端(rapid amplification of cDNA ends,RACE)技术首次克隆了翘嘴鲌TMEM-57蛋白基因的cDNA全序列,该序列全长为2822bp,其中5′-UTR区93bp,3′-UTR区731bp,开放阅读框(open reading frame,ORF)为1998bp,编码665个氨基酸。NJ法系统进化分析将同科或属中TMEM-57基因分成一个分支,表现出种属间的高度保守性。利用荧光定量PCR(qPCR)技术检测了不同组织的表达量,发现在卵巢中表达量最高,其次为心脏和脑。用直接测序法对TMEM-57基因部分区段单核苷酸多态性(SNP)进行了检测,寻找到11个新的SNP位点。In this paper, a 2822bp full-length cDNA sequence of TMEM-57 gene from Culter alburnus was obtained with RT-PCR and rapid amplification of eDNA ends （RACE） technique. It consists of a 93bp 5＇ untranslated region （UTR）, an 1998bp open reading frame （ORF） and a 731bp 3＇UTR. The translated protein is composed of 665 amino acids. Neighbor-Joining （NJ） tree suggested that the TMEM-57 gene is highly conservative between species clustering the same species. After detected the gene expression quantity in tissues with real-time fluorescence quantitative PCR （qPCR）, expression quantity in the ovary is considered highest, then the heart and brain. Certain fragments of TMEM-57 are inspected for single nucleotide polymorphism （SNP） by sequencing of PCR production, eleven novel SNP loci are detected.

关 键 词：翘嘴鲌 TMEM-57 荧光定量PCR 单核苷酸多态性 

分 类 号：Q78[生物学—分子生物学]