酚红法和改良重量法分别研究葛根素的大鼠在体肠吸收机制  

Transport of puerarin in rat intestine in situ by modified gravimetry and phenol red assay

作　　者：黄嗣航[1] 龙晓英[1] 袁飞[1] 陈莉[1] 蔡宝玲[1] 邱慧琳[1] 

机构地区：[1]广东药学院中药学院,广东广州510006

出　　处：《广东药学院学报》2012年第6期603-607,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金面上项目(30973953C1909)

摘　　要：目的通过在体单向肠灌流法研究葛根素的肠吸收机制,比较酚红法与改良重量法在研究葛根素吸收机制上的适用性。方法采用酚红法、改良重量法校正灌流液体积,并用HPLC法对灌流前后葛根素的含量进行测定,研究不同质量浓度(100、200和400μg.mL-1)的葛根素在大鼠不同肠段的吸收情况。结果酚红法测得的葛根素的吸收参数基本为负值,其结果不符合常理。改良重量法测得葛根素在各肠段均有吸收,但各肠段之间差异无统计学意义,不存在明显吸收窗;葛根素浓度升高,肠吸收有所增加,且不存在饱和现象。结论改良重量法相对酚红法更适用于葛根素的在体肠吸收研究。Objective To study the rat intestine absorption mechanism of puerarin by in situ single pass perfusion,and compare the difference of gravimetry and phenol red assay.Methods The rat single pass perfusion technique was applied.Gravimetry and phenol red assay were used to correct the perfusion volume.The concentration of puerarin was determined by HPLC.A series of studies were carried out including the absorption at different concentrations（100,200 and 400 ·mL^-1） and at different intestinal regions.Results Phenol red assay′s parameters were almost all of negative values.These results went against common sense.Gravimetry′s results showed that puerarin could be absorbed in all rat intestines without special absorption sites and the differences were negligible.There was no highest saturated concentration raised with the increasing concentration of puerarin.Conclusion Gravimetry was more suitable than phenol red assay for studying the absorption mechanism of puerarin.

关 键 词：葛根素 在体单向肠灌流法 吸收机制 重量法 酚红法 

分 类 号：R285[医药卫生—中药学]