人16周胎儿脑cDNA文库的构建及鉴定  

Construction and analysis of cDNA library of human 16-weeks-old fetal brain.

作　　者：杨岐生[1] 林卿[1] 谢毅[2] 

机构地区：[1]浙江大学生物科学与技术系,浙江杭州310027 [2]复旦大学遗传学国家重点实验室,上海200433

出　　处：《浙江大学学报（理学版）》2000年第3期320-323,共4页Journal of Zhejiang University（Science Edition）

基　　金：国家自然科学基金!(39270 370);复旦大学遗传学国家重点实验室基金资助项目

摘　　要：本文从 16周的胎儿脑中抽提总 m RNA,经过一系列酶促反应后合成 c DNA,分级分离柱除去小片段 DNA后克隆到λgt10载体中 ,转染宿主菌 C60 0 hfl,文库包装效率为 4 .2× 10 6pfu/μg,得到的原始克隆数为 2 .1× 10 6,c DNA插入片段平均大于 1.2 kpb.根据已知序列设计引物 ,从这个 c DNA文库中扩增出血管内皮生长因子 ( VEGF)的全长 cTotal mRNA was extracted from a 16\|weeks\|old human fetal brain. The cDNAs were synthesized after a series of enzyme\|catalyzed reaction and then cloned into the λgt10 vector after removing short segments by cDNA size fractionation columns. The efficiency of packaging is about 4.2×10\+6 pfu/μg after infecting the host \%C600hfl\%. The average length of the inserted cDNA is about 1.2 kpb. The VEGF full\|length gene from the cDNA library by PCR with primers designed in base of the gene sequence of vascular endothelial growth factor was amplified.

关 键 词：胚胎脑 CDNA文库 脑基因 VEGF 胎儿 构建 鉴定 

分 类 号：Q987[生物学—遗传学] R321[生物学—人类学]