检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:杨静[1] 丁莉坤[1] 王钊[2] 李健康[1] 冯智军[1] 杨莉[3] 贾艳艳[1] 文爱东[1]
机构地区:[1]第四军医大学西京医院药剂科,西安710032 [2]第四军医大学预防医学系毒理学教研室 [3]三峡大学医学院
出 处:《中国药师》2012年第12期1688-1692,共5页China Pharmacist
摘 要:目的:评价液质联用(HPLC-MS/MS)测定人血浆中来曲唑浓度方法的测量不确定度方法:对HPLC-MS/MS法测定血浆中来曲唑浓度的全过程进行分析分析测量不确定度的来源,用A类评定程序评价分析过程中随机效应引起的不确定度,用B类评定程序评价其他因素引起的不确定度,量化各个不确定度分量,最后根据各分量计算出合成标准测量不确定度,并给出扩展测量不确定度报告结果:置信概率P为95%时,血浆高(79.28 ng·ml^(-1))、中(8.378 ng·ml^(-1))、低(0.099 97 ng·ml^(-1))三个浓度的扩展不确定度分别为3 99,0.417,0.035 32 ng·ml^(-1)。结论:本方法不确定度主要来自标准曲线的拟合过程,其适用于HPLC-MS/MS法测定血浆来曲唑的不确定度评定,为用HPLC-MS/MS法测定血浆浓度的不确定度评定提供了重要参考。Objective:To evaluate the uncertainty of the letrozole determination in human plasma hy HPLC-MS/MS.Method;The uncertainty sources were obtained from the whole procedure analysis of HPLC-MS/MS method.The uncertainty caused by random effects was evaluated with type A and the others were by type B.Each active components of the uncertainty was calculated,and the extended uncertainty was obtained.Result;The expanded uncertainty for low(0.099 97 ng ? ml^(-1)),medium(8.378 ng ? ml^(-1) ) and high(79.28 ng?ml^(-1) ) level of letrozole were 0.035 32,0.417 and 3.99 ng ? ml^(-1),respectively(P=95%).Conclusion:The uncertainty of the method is mainly caused by the calibration curve.The method is applicable in the uncertainty evaluation of the letrozole deteiTnination in human plasma.It provides important reference for the uncertainty evaluation of the drug determination in human plasma by HPLC-MS/MS.
分 类 号:R917[医药卫生—药物分析学]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.179