冬葵愈伤组织诱导技术研究  

Study on the Callus Induction Technology of Malva verticillata L.

作　　者：黄凤兰[1] 赵永[1] 布和巴特[2] 李海峰[1] 卜志刚[1] 李跃[1] 曾健寒[1] 邹千稳[1] 陈玲玲[1] 

机构地区：[1]内蒙古民族大学生命科学学院,内蒙古通辽028000 [2]内蒙古民族大学,蒙医药学院内蒙古通辽028000

出　　处：《内蒙古农业科技》2012年第6期24-25,共2页Inner Mongolia Agricultural Science and Technology

基　　金：内蒙古民族大学教育教学研究项目(No.2009053)

摘　　要：冬葵(Malva verticillata L.)是一种具有很高药用价值的蒙药,实验以冬葵叶片和茎段为外植体诱导愈伤组织,为冬葵的快繁和细胞培养奠定基础。实验结果如下:冬葵叶片和茎段最适消毒条件是0.1%升汞分别消毒50s、2.5min;叶片和茎段诱导愈伤组织阶段最适培养基相同,均为MS+蔗糖(30g/L)+琼脂(7g/L)+NAA(0.4mg/L)+6-BA(3mg/L)+抗坏血酸(600mg/L)。Malva verticillata L.is a valuable medicinal plant in Mongolian pharmacy.In this paper,we carried out callus induction experiments with leaves and stem sections from this plant as explants,and laid a solid foundation for future rapid propagation and cell culture.The optimized experiment protocols were as follows.The leaves and stems were sterilized in HgCl 2 for 50 s or 2.5 min,and then placed on the developed medium containing MS,30g/L sucrose,7g/L Agar,0.4mg/L NAA,3mg/L 6-BA and 200mg/ L vitamin C.

关 键 词：冬葵 愈伤组织 植物生长调节剂 

分 类 号：S567[农业科学—中草药栽培]