利用有机溶剂法快速纯化绿色荧光蛋白  

Rapid Purification of Green Fluorescent Protein from Escherichia Coli by Organic Extraction

作　　者：王盛[1,2] 董洁[1] 曾瑾[1,2] 曹慜[1] 张虹[1] 雒丽娜[1] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]宁夏大学 西部特色生物资源保护与利用教育部重点实验室,宁夏银川750021

出　　处：《宁夏大学学报（自然科学版）》2012年第4期400-404,共5页Journal of Ningxia University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31060023);宁夏回族自治区大学生创新基金资助项目

摘　　要：介绍非色谱GFP纯化方法——有机溶剂纯化法.该方法在短时间内(30min)即可获得大量较高纯度(91.7%),且荧光光谱特征不改变的GFP蛋白.纯化过程简单,无需昂贵的仪器和设备,适合绝大多数中小实验室使用.Green fluorescent protein （GFP） is widely used as an excellent reporter molecule and cell biology. Some biochemical and immunological assays require high-purity GFP. in biochemistry However, the majority of current procedures for GFP purification includes multiple time-consuming chromatography steps with a low yield of the desired product or requires tag-containing proteins. In the present work, an alternative method is described for the GFP purification using organic extraction. The method is easily scalable for yielding a highly homogeneous GFP protein indistinguishable in spectroscopic properties from the origin one.

关 键 词：绿色荧光蛋白 表达与纯化 有机提取 两相系统 

分 类 号：Q78[生物学—分子生物学] Q78