创伤弧菌外膜蛋白免疫刺激复合物最小免疫剂量测定  

Minimum Dosage of Vibrio vulnifiticus OMP-ISCOMs for Vaccinating Anguilla anguilla

作　　者：许斌福[1] 龚晖[1] 陈秀锦[2] 田丁[1] 陈强[1] 陈永聪[1] 林天龙[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003 [2]福建省疾病预防控制中心,福建福州350001

出　　处：《福建农业学报》2012年第12期1280-1282,共3页Fujian Journal of Agricultural Sciences

基　　金：福建省农业科学院科技创新团队建设项目(STIF-Y02);福建省科技创新平台项目(2009N2003);国家自然科学基金项目(31101933);福建省农业科学院科研项目(工程化实验室类);福建省科技计划项目--省属公益类科研院所基本科研专项(2010R1021-5)

摘　　要：为探讨创伤弧菌Vibrio vulnifiticus外膜蛋白(outer membrane protein,OMP)免疫刺激复合物(Immune stimulating Complexs,ISCOMs)的抗原性,制备创伤弧菌OMP-ISCOMs腹腔注射免疫欧洲鳗鲡Anguilla anguilla(平均规格为17g·尾-1),通过免疫血清学检测和攻毒保护试验,测定ISCOMs最小免疫剂量。结果表明,于免疫后第30d用FJ03-X2菌株攻毒,免疫组40、20、10、5μg·尾-1的免疫保护力分别为100%、87.5%、75%、50%;免疫血清效价分别为1∶6 400、1∶3 200、1∶800、1∶200。结果说明创伤弧菌外膜蛋白ISCOMs的最小免疫剂量为0.59μg.g-1水温(24±1)℃,并证实了创伤弧菌OMP-ISCOMs具强抗原性。Antigenicity of the immune stimulating complexes in the outer membrane protein（OMP-ISCOMs） of Vibrio vulnifiticus was studied by injecting the vaccine into the European eels（Anguilla anguilla）.The minimum dosage required for effective vaccination was determined by serology and immune protection test.The immune response was dosage dependent.Thirty days after the eels were challenged with the V.vulnificus FJ03-X2 strains at 200,100,50 and 25 μg·mL-1 to result in 100%,87.5%,75% and 50% of protection,respectively;and the titers of serum antibody of 1∶6400,1∶3200,1∶800 and 1∶200 in turn,respectively.It appeared that the minimum dose of the OMP-ISCOMs of Vibrio vulnifiticus vaccine was 0.59 μg·g-1.

关 键 词：创伤弧菌 OMP-ISCOMs 最小免疫剂量 欧洲鳗鲡 

分 类 号：S942.3[农业科学—水产养殖]